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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1692
Title: Nitrilase-catalysed conversion of acrylonitrile by free and immobilized cells of Streptomyces sp.
Authors: Nigam V.K.
Khandelwal A.K.
Gothwal R.K.
Mohan M.K.
Choudhury B.
Vidyarthi A.S.
Ghosh P.
Published in: Journal of Biosciences
Abstract: The biotransformation of acrylonitrile was investigated using thermophilic nitrilase produced from a new isolate Streptomyces sp. MTCC 7546 in both the free and immobilized state. Under optimal conditions, the enzyme converts nitriles to acids without the formation of amides. The whole cells of the isolate were immobilized in agar-agar and the beads so formed were evaluated for 25 cycles at 50°C. The enzyme showed a little loss of activity during reuse. Seventy-one per cent of 0.5 M acrylonitrile was converted to acid at 6 h of incubation at a very low density of immobilized cells, while 100% conversion was observed at 3 h by free cells. © 2009 Indian Academy of Sciences.
Citation: Journal of Biosciences (2009), 34(1): 21-26
URI: https://doi.org/10.1007/s12038-009-0005-7
http://repository.iitr.ac.in/handle/123456789/1692
Issue Date: 2009
Keywords: Aliphatic nitriles
Biotransformation
Immobilization
Nitrilase
Streptomyces
Thermophilic
ISSN: 2505991
Author Scopus IDs: 7004690752
18433918500
12242620500
12242902600
7103333522
26641549500
57212696908
Author Affiliations: Nigam, V.K., Birla Institute of Scientific Research, Statue Circle, Jaipur 302 001, India
Khandelwal, A.K., Birla Institute of Scientific Research, Statue Circle, Jaipur 302 001, India
Gothwal, R.K., Birla Institute of Scientific Research, Statue Circle, Jaipur 302 001, India
Mohan, M.K., Birla Institute of Scientific Research, Statue Circle, Jaipur 302 001, India
Choudhury, B., Faculty of Biological Science, Indian Institute of Technology, Roorkee 247 667, India
Vidyarthi, A.S., Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi 835 215, India
Ghosh, P., Birla Institute of Scientific Research, Statue Circle, Jaipur 302 001, India
Funding Details: One of the authors (AKK) wishes to acknowledge the financial assistance received from Indian Council of Medical Research, New Delhi, for carrying out this work.
Corresponding Author: Nigam, V. K.; Birla Institute of Scientific Research, Statue Circle, Jaipur 302 001, India; email: nigam.bisr@gmail.com
Appears in Collections:Journal Publications [BT]

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