http://repository.iitr.ac.in/handle/123456789/1674| Title: | Anti-androgenic endocrine disrupting activities of chlorpyrifos and piperophos |
| Authors: | Viswanath G. Chatterjee S. Dabral S. Nanguneri S.R. Divya G. Roy, Partha |
| Published in: | Journal of Steroid Biochemistry and Molecular Biology |
| Abstract: | The present work describes the screening and characterization of some common endocrine disrupting chemicals for their (anti)androgenic activities. Various chemicals (mostly pesticides and pharmaceuticals) were screened with the NIH3T3 cell line stably expressing human androgen receptor (hAR) and luciferase reporter gene for their ability to stimulate luciferase activity or inhibit the response that was evoked by 0.4. nM testosterone. The most potent anti-androgenic compounds identified in our assay included chlorpyrifos, endosulfan and piperophos. Finally, the chemicals were analyzed for their effects on steriodogenesis in rat Leydig cells. Piperophos and chlorpyrifos showed a significant decrease in testosterone biosynthesis by Leydig cells. RT-PCR studies showed decrease in the expression of key steroidogenic enzymes: cytochrome P450scc, 3?-HSD and 17?-HSD and immunoblot analysis demonstrated a decrease in steroidogenic acute regulatory (StAR) protein expression by both these chemicals. Chlorpyrifos also showed a decrease in LH receptor stimulated cAMP production. In conclusion, we demonstrate that commonly used pesticides like chlorpyrifos and piperophos pose serious threat to male reproductive system by interfering at various levels of androgen biosynthesis. © 2010 Elsevier Ltd. |
| Citation: | Journal of Steroid Biochemistry and Molecular Biology (2010), 120(1): 22-29 |
| URI: | https://doi.org/10.1016/j.jsbmb.2010.02.032 http://repository.iitr.ac.in/handle/123456789/1674 |
| Issue Date: | 2010 |
| Keywords: | Androgen receptor Anti-androgenic Endocrine disruptors StAR Steroidogenic enzymes |
| ISSN: | 9600760 |
| Author Scopus IDs: | 23037156500 17341895000 57065165000 35759472100 25222813300 35509207200 |
| Author Affiliations: | Viswanath, G., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India, Department of Medicine, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT, United States Chatterjee, S., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India Dabral, S., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India, International Max Plank Research School for Heart and Lung Research, Parstrasse 1, 61231 Bad Nauheim, Germany Nanguneri, S.R., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India, MaxPlank Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany Divya, G., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India Roy, P., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India |
| Funding Details: | The authors would like to thank Professor Ilpo Huhtaniemi, Imperial College London, UK for kindly providing all steroidal and non-steroidal test chemicals and expression vectors and Professor D.M. Stocco of Texas Technical University, USA, for kindly providing StAR antibodies. The study was supported by the Department of Biotechnology (DBT, no. BT/BCE/08/355/04), Government of India and Council of Scientific and Industrial Research (CSIR, no. 37(1200)/04/EMR II), Government of India, as funded projects to P.R., fellowships to G.V. and S.C. from DBT and CSIR, respectively. The authors would thus like to thank both these funding agencies. |
| Corresponding Author: | Roy, P.; Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India; email: paroyfbs@iitr.ernet.in |
| Appears in Collections: | Journal Publications [BT] |
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