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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1673
Title: Nitrile-metabolizing potential of Amycolatopsis sp. IITR215
Authors: Babu V.
Shilpi
Choudhury, Bijan
Published in: Process Biochemistry
Abstract: Strain Amycolatopsis sp. IITR215 was isolated from a sewage sample using polyacrylonitrile powder as the sole nitrogen source. Identification was performed by 16S rDNA analysis. The isolated strain harbored multiple nitrile-metabolizing enzymes having a wide range of substrate specificities. It metabolized nitrile and amide compounds with constitutive enzymes. Studies using an amidase inhibitor showed that hydrolysis of acrylonitrile and acrylamide occurred due to nitrile hydratase and amidase, respectively, while hydrolysis of hexanenitrile was due to the action of either nitrilase or a second nitrile hydratase/amidase system. The inhibitory effects of N-bromosuccinimide and N-ethylmaleimide on enzymes of this culture were also studied and this further indicated the involvement of either a nitrilase or a second nitrile hydratase/amidase system for hydrolysis of hexanenitrile. Interestingly, hexanenitrile hydrolysis exhibited an optimum temperature of 55 °C, whereas acrylonitrile and acrylamide hydrolysis showed an optimum temperature of 45 °C. The optimum pH was 5.8 for hexanenitrile hydrolysis and 7.0 for acrylonitrile and acrylamide hydrolysis. Hexanenitrile hydrolysis by enzymes of this strain showed better organic solvent tolerance in the presence of alcohols. The maximum enzyme activity of nitrile-metabolizing enzymes was found using media containing isobutyramide as the nitrogen source. This is the first report on constitutive multiple enzymes from the Amycolatopsis genus. © 2010 Elsevier Ltd. All rights reserved.
Citation: Process Biochemistry (2010), 45(6): 866-873
URI: https://doi.org/10.1016/j.procbio.2010.02.008
http://repository.iitr.ac.in/handle/123456789/1673
Issue Date: 2010
Keywords: Amycolatopsis sp. IITR215
Diethylphosphoramidate (DEPA)
Hexanenitrile
Nitrilase
Nitrile hydratase/amidase
Polyacrylonitrile
ISSN: 13595113
Author Scopus IDs: 8982627200
36671975400
7103333522
Author Affiliations: Babu, V., Department of Biotechnology, Indian Institute of Technology, IIT Roorkee, Roorkee-247667, Uttarakhand, India
Shilpi, Department of Biotechnology, Indian Institute of Technology, IIT Roorkee, Roorkee-247667, Uttarakhand, India
Choudhury, B., Department of Biotechnology, Indian Institute of Technology, IIT Roorkee, Roorkee-247667, Uttarakhand, India
Funding Details: The authors gratefully acknowledge financial support from the Department of Biotechnology, University of Pune , which provided the junior research fellowship to Vikash Babu.
Corresponding Author: Choudhury, B.; Department of Biotechnology, Indian Institute of Technology, IIT Roorkee, Roorkee-247667, Uttarakhand, India; email: bijanfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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