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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1668
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dc.contributor.authorRani R.-
dc.contributor.authorGhosh S.-
dc.date.accessioned2020-09-30T11:39:38Z-
dc.date.available2020-09-30T11:39:38Z-
dc.date.issued2011-
dc.identifier.citationBioresource Technology (2011), 102(22): 10641-10649-
dc.identifier.issn9608524-
dc.identifier.other21945206-
dc.identifier.urihttps://doi.org/10.1016/j.biortech.2011.08.075-
dc.identifier.urihttp://repository.iitr.ac.in/handle/123456789/1668-
dc.description.abstractPresent study introduces linseed oil cake as a novel substrate for phytase production by Rhizopus oryzae. Statistical approach was employed to optimize various medium components under solid state fermentation (SSF). An overall 8.41-fold increase in phytase production was achieved at the optimum concentrations (w/w, mannitol, 2.05%; ammonium sulfate, 2.84% and phosphate, 0.38%). Further enhancement by 59% was observed due to a novel strain improvement approach. Purified phytase (?34kDa) showed optimal temperature of 45°C, dual pH optima at 1.5 and 5.5 and possesses high catalytic efficiency (2.38×10 6M -1s -1). Characterization study demonstrates the phytase as highly thermostable and resistant to proteolysis, heavy metal ions, etc. Furthermore, an improved HPLC method was introduced to confirm the ability of phytase to degrade phytic acid completely and was found to be an efficient method. © 2011.-
dc.language.isoen_US-
dc.relation.ispartofBioresource Technology-
dc.subjectLinseed oil cake-
dc.subjectOptimization-
dc.subjectPhytase-
dc.subjectRhizopus oryzae-
dc.subjectStrain improvement-
dc.titleProduction of phytase under solid-state fermentation using Rhizopus oryzae: Novel strain improvement approach and studies on purification and characterization-
dc.typeArticle-
dc.scopusid55773009800-
dc.scopusid56603000600-
dc.affiliationRani, R., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India-
dc.affiliationGhosh, S., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India-
dc.description.fundingThe authors greatly appreciate the financial assistance in the form of junior research fellowship by University Grants Commission, New Delhi, India .-
dc.description.correspondingauthorGhosh, S.; Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India; email: ghoshfbs@iitr.ernet.in-
Appears in Collections:Journal Publications [BT]

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