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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1668
Title: Production of phytase under solid-state fermentation using Rhizopus oryzae: Novel strain improvement approach and studies on purification and characterization
Authors: Rani R.
Ghosh S.
Published in: Bioresource Technology
Abstract: Present study introduces linseed oil cake as a novel substrate for phytase production by Rhizopus oryzae. Statistical approach was employed to optimize various medium components under solid state fermentation (SSF). An overall 8.41-fold increase in phytase production was achieved at the optimum concentrations (w/w, mannitol, 2.05%; ammonium sulfate, 2.84% and phosphate, 0.38%). Further enhancement by 59% was observed due to a novel strain improvement approach. Purified phytase (?34kDa) showed optimal temperature of 45°C, dual pH optima at 1.5 and 5.5 and possesses high catalytic efficiency (2.38×10 6M -1s -1). Characterization study demonstrates the phytase as highly thermostable and resistant to proteolysis, heavy metal ions, etc. Furthermore, an improved HPLC method was introduced to confirm the ability of phytase to degrade phytic acid completely and was found to be an efficient method. © 2011.
Citation: Bioresource Technology (2011), 102(22): 10641-10649
URI: https://doi.org/10.1016/j.biortech.2011.08.075
http://repository.iitr.ac.in/handle/123456789/1668
Issue Date: 2011
Keywords: Linseed oil cake
Optimization
Phytase
Rhizopus oryzae
Strain improvement
ISSN: 9608524
Author Scopus IDs: 55773009800
56603000600
Author Affiliations: Rani, R., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India
Ghosh, S., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India
Funding Details: The authors greatly appreciate the financial assistance in the form of junior research fellowship by University Grants Commission, New Delhi, India .
Corresponding Author: Ghosh, S.; Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India; email: ghoshfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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