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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1660
Title: Optimization of medium composition for enhanced chitin deacetylase production by mutant Penicillium oxalicum SAEM-51 using response surface methodology under submerged fermentation
Authors: Pareek N.
Singh R.P.
Ghosh S.
Published in: Process Biochemistry
Abstract: Nutritional requirement for maximum production of chitin deacetylase (CDA), an enzyme used for bioconversion of chitin to chitosan, under submerged condition by mutant Penicillium oxalicum SAEM-51 was optimized using response surface methodology (RSM). Among the eight nutritional elements studied, yeast-extract, peptone, (NH4)2SO4 and MgSO4 were identified as the most critical variables for CDA production by Plackett-Burman design first. Further optimization of these variables was done by using a four factor central composite design (CCD). The model came out to be significant and statistical analysis (analysis of variance, ANOVA) of the results showed that yeast extract had contributed significantly (quadratic effect) to the response and its interaction with magnesium sulfate was strong. Peptone was also found to be another important component in the medium that influence the CDA production. Optimum concentration of the variables for enhanced CDA production determined by CCD were - yeast extract (X 1) 3.165 g l-1, peptone (X2) 4.754 g l -1, (NH4)2SO4 (X4) 0.255 g l-1 and MgSO4 (X5) 0.535 g l-1. 2.0 fold enhancement in CDA titres was attained in shake flasks, when the variables were used at their optimum levels. CDA production (414.7 ± 6.32 U l-1) in the optimized medium was in good agreement with the values predicted by the quadratic model (399.08 U l-1), confirming the validity of the model. Employment of optimal growth medium would result into large scale production of microbial deacetylase, which further heighten its potential for biological production of chitosan. © 2011 Elsevier Ltd.
Citation: Process Biochemistry (2011), 46(8): 1693-1697
URI: https://doi.org/10.1016/j.procbio.2011.05.002
http://repository.iitr.ac.in/handle/123456789/1660
Issue Date: 2011
Keywords: Central composite design
Chitin deacetylase
Optimization
Penicillium oxalicum
Plackett-Burman design
ISSN: 13595113
Author Scopus IDs: 35741588300
57208931324
56603000600
Author Affiliations: Pareek, N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee -247 667, Uttarakhand, India
Singh, R.P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee -247 667, Uttarakhand, India
Ghosh, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee -247 667, Uttarakhand, India
Corresponding Author: Ghosh, S.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee -247 667, Uttarakhand, India; email: ghoshfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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