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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1656
Title: Crystallization, high-resolution data collection and preliminary crystallographic analysis of Aura virus capsid protease and its complex with dioxane
Authors: Aggarwal M.
Dhindwal S.
Pratap S.
Kuhn R.J.
Kumar, Pravindra R.Manish
Tomar, Shailly
Published in: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Abstract: The C-terminal protease domain of capsid protein from Aura virus expressed in a bacterial expression system has been purified to homogeneity and crystallized. Crystals suitable for X-ray diffraction analysis were obtained by the vapour-diffusion method using 0.1 M bis-tris and polyethylene glycol monomethyl ether 2000. Crystals of the C-terminal protease domain of capsid protein in complex with dioxane were also produced and crystal data were obtained. Both crystals belonged to space group C2, with unit-cell parameters a = 79.6, b = 35.2, c = 49.5 Å. High-resolution data sets were collected to a resolution of 1.81 Å for the native protein and 1.98 Å for the complex. Preliminary crystallographic studies suggested the presence of a single molecule in the crystallographic asymmetric unit, with a solvent content of 38.5%. © 2011 International Union of Crystallography. All rights reserved.
Citation: Acta Crystallographica Section F: Structural Biology and Crystallization Communications(2011), 67(11): 1394-1398
URI: https://doi.org/10.1107/S174430911103404X
http://repository.iitr.ac.in/handle/123456789/1656
Issue Date: 2011
Keywords: Aura virus
capsid protease
ISSN: 17443091
Author Scopus IDs: 54411866100
36082537700
54412938100
7202460038
55064809000
57203506001
Author Affiliations: Aggarwal, M., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247 667, India
Dhindwal, S., Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247 667, India
Pratap, S., Department of Biotechnolo
Corresponding Author: Tomar, S.; Department of Biotechnology, Indian Institute of Technology, Roorkee, Roorkee 247 667, India; email: shailfbt@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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