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Title: Use of an osmotically sensitive mutant of Propionibacterium freudenreichii subspp. shermanii for the simultaneous productions of organic acids and trehalose from biodiesel waste based crude glycerol
Authors: Ruhal R.
Choudhury, Bijan
Published in: Bioresource Technology
Abstract: Recently suitability of crude glycerol for trehalose and propionic acid productions was reported using Propionibacterium freudenreichii subspp. shermanii and it was concluded that presence of KCl in crude glycerol was the probable reason for higher trehalose accumulation with crude glycerol medium. To further improve trehalose production, an osmotic sensitive mutant of this strain (non-viable in medium with 3% NaCl) with higher trehalose yield was isolated. In mutant, trehalose yields achieved with respect to biomass and substrate consumed (391. mg/g of biomass, 90. mg/g of substrate consumed) were three and four times higher, respectively as compared to parent strain when crude glycerol was used as a carbon source. Other major fermentation products obtained were propionic acid (0.42. g/g of substrate consumed) and lactic acid (0.3. g/g of substrate consumed). It was also observed that in mutant higher activity of ADP-glucose pyrophosphorylase was probably responsible for higher trehalose accumulation. © 2012 Elsevier Ltd.
Citation: Bioresource Technology (2012), 109(): 131-139
Issue Date: 2012
Keywords: Crude glycerol
Osmotic sensitive mutant
Propionibacterium freudenreichii subspp. shermanii
ISSN: 9608524
Author Scopus IDs: 54411237100
Author Affiliations: Ruhal, R., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT), Roorkee, India
Choudhury, B., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT), Roorkee, India
Funding Details: This work was supported by Department of Biotechnology (DBT), Govt. of India, with Grant No. BT/PR-7493/PID/20/289/2006.
Corresponding Author: Choudhury, B.; Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttrakhand 247667, India; email:
Appears in Collections:Journal Publications [BT]

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