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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1632
Title: Purification and characterization of chitin deacetylase from Penicillium oxalicum SAE M-51
Authors: Pareek N.
Vivekanand V.
Saroj S.
Sharma A.K.
Singh R.P.
Published in: Carbohydrate Polymers
Abstract: Chitin deacetylase (CDA) with a molecular mass of 53 kDa was purified from Penicillium oxalicum SAE M-51. Optimal temperature and pH of the purified enzyme were 50 °C and 9.0 respectively. Activation energy (E a,d), free energy (?Gd), enthalpy (?Hd) and entropy (?Sd) for enzyme denaturation at optimal temperature were 114.72 kJ mol -1, 97.86 kJ mol -1, 112.04 kJ mol -1, 43.93 J mol -1 K -1, respectively. Enzyme had the half life of 693.10 min at its optimum temperature. It had notably deacetylated glycol chitin and chitin oligomers having degree of polymerization of more than four. Increased enzyme activity was observed with metal ions mainly Cu 2+ and Co 2+. No inhibition of the enzyme was observed by the end product i.e. acetate (0-60 mM). Far-UV CD spectroscopic analysis revealed presence of 56.26% ? and 15.63% ?-helical structures. Significant homology of the enzyme was observed with CDAs from fungal and yeast strains. © 2011 Elsevier Ltd. All rights reserved.
Citation: Carbohydrate Polymers(2012), 87(2): 1091-1097
URI: https://doi.org/10.1016/j.carbpol.2011.08.041
http://repository.iitr.ac.in/handle/123456789/1632
Issue Date: 2012
Keywords: Chitin deacetylase
Circular dichroism
Kinetics
Penicillium oxalicum
Purification
Thermodynamic parameters
ISSN: 1448617
Author Scopus IDs: 35741588300
57211114758
36121053900
57214355701
57208931324
Author Affiliations: Pareek, N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India
Vivekanand, V., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India
Saroj, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India
Sharma, A.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India
Singh, R.P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India
Funding Details: Senior research fellowship awarded to NP by Council of Scientific and Industrial Research, New Delhi, India and Junior research fellowship awarded to SS by Department of Biotechnology, New Delhi, India are gratefully acknowledged.
Corresponding Author: Singh, R.P.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India; email: rpsbsfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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