Skip navigation
Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1615
Title: Abnormal endosperm development causes female sterility in rice insertional mutant OsAPC6
Authors: Awasthi A.
Paul P.
Kumar S.
Verma S.K.
Prasad R.
Dhaliwal H.S.
Published in: Plant Science
Abstract: A T-DNA insertional mutant OsAPC6 of rice, with gibberellic acid insensitivity and reduced height, had up to 45% reduced seed set. The insertion occurred on chromosome 3 of rice in the gene encoding one of the subunits of anaphase promoting complex/Cyclosome APC6. The primary mother cells of the mutant plants had normal meiosis, male gametophyte development and pollen viability. Confocal laser scanning microscopic (CLSM) studies of megagametophyte development showed abnormal mitotic divisions with reduced number or total absence of polar nuclei in about 30-35% megagametophytes of OsAPC6 mutant leading to failure of endosperm and hence embryo and seed development. Abnormal female gametophyte development, high sterility and segregation of tall and gibberellic acid sensitive plants without selectable marker Hpt in the selfed progeny of OsAPC6 mutant plants indicate that the mutant could be maintained in heterozygous condition. The abnormal mitotic divisions during megagametogenesis could be attributed to the inactivation of the APC6/CDC16 of anaphase promoting complex of rice responsible for cell cycle progression during megagametogenesis. Functional validation of the candidate gene through transcriptome profiling and RNAi is in progress. © 2011 Elsevier Ireland Ltd.
Citation: Plant Science (2012), 183(): 167-174
URI: https://doi.org/10.1016/j.plantsci.2011.08.007
http://repository.iitr.ac.in/handle/123456789/1615
Issue Date: 2012
Keywords: Anaphase promoting complex
Confocal microscopy
Megagametogenesis
T-DNA insertional mutant
ISSN: 1689452
Author Scopus IDs: 37014402100
7202714050
55500537400
55303446800
16690394400
35466824500
Author Affiliations: Awasthi, A., Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India
Paul, P., Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India
Kumar, S., Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India
Verma, S.K., Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India
Prasad, R., Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India
Dhaliwal, H.S., Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India
Funding Details: The authors are grateful to Dr. Akhshay Pradhan, Department of Genetics, South Campus, Delhi University, for providing laboratory facilities in the present investigation. The lead author is thankful to the Council of Scientific and Industrial Research, Government of India, for providing financial assistance in the form of Research Fellowship (EU-IV/2008/June/318579). The authors are also grateful to the Department of Biotechnology, Govt. of India for funding the project.
Corresponding Author: Awasthi, A.; Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India; email: anjkam.awasthi@gmail.com
Appears in Collections:Journal Publications [BT]

Files in This Item:
There are no files associated with this item.
Show full item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.