Skip navigation
Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1614
Title: Competitive adsorptions of nitrile hydratase and amidase on polyacrylonitrile and its effect on surface modification
Authors: Babu V.
Choudhury B.
Published in: Colloids and Surfaces B: Biointerfaces
Abstract: In this study, enzymatic surface modification of polyacrylonitrile was studied using nitrile metabolizing enzyme of Amycolatopsis sp. IITR 215. During enzymatic treatment of polyacrylonitrile at pH of 5.8 and 7, it was observed that the conversion of cyano group to carboxylic acid at pH 5.8 was three times higher than at pH 7. This difference in enzymatic treatment efficiency was explained by studying the differences in adsorption profiles of nitrile hydratase and amidase on polyacrylonitrile at pH of 5.8 and 7. Adsorption profiles were determined by monitoring the unbound activities of these two enzymes in the supernatant. From the specific activity profiles of bound nitrile hydratase and amidase it was concluded that more specific binding of nitrile hydratase was observed at pH 5.8 as compared to pH 7. In case of amidase, optimum adsorption was obtained at pH 5.8 within 5. h whereas in case of pH 7 it was obtained within 20. h. Thus at pH 7, sequential adsorption of nitrile hydratase and amidase was observed and this adsorption profile was similar to the Vroman effect reported during plasma protein adsorption at solid-liquid interface. Ideally, specific nitrile hydratase adsorption followed by sequential adsorption of amidase may enhance higher conversion of cyano group to carboxylic acid. © 2011 Elsevier B.V.
Citation: Colloids and Surfaces B: Biointerfaces (2012), 89(1): 277-282
URI: https://doi.org/10.1016/j.colsurfb.2011.08.025
http://repository.iitr.ac.in/handle/123456789/1614
Issue Date: 2012
Keywords: Amidase
Nitrile hydratase
Polyacrylonitrile
Specific adsorption
Vroman effect
ISSN: 9277765
Author Scopus IDs: 8982627200
7103333522
Author Affiliations: Babu, V., Department of Biotechnology, Indian Institute of Technology, Roorkee 247 667, India
Choudhury, B., Department of Biotechnology, Indian Institute of Technology, Roorkee 247 667, India
Funding Details: The authors gratefully acknowledge the financial support from the Department of Biotechnology, Govt. of India for providing research fellowship to Vikash Babu.
Corresponding Author: Choudhury, B.; Department of Biotechnology, Indian Institute of Technology, Roorkee 247 667, India; email: bijanfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

Files in This Item:
There are no files associated with this item.
Show full item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.