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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1611
Title: Improved trehalose production from biodiesel waste using parent and osmotically sensitive mutant of Propionibacterium freudenreichii subsp. shermanii under aerobic conditions
Authors: Ruhal R.
Choudhury B.
Published in: Journal of Industrial Microbiology and Biotechnology
Abstract: Trehalose is an important nutraceutical of wide commercial interest in the food processing industry. Recently, crude glycerol was reported to be suitable for the production of trehalose using a food microbe, Propionibacterium freudenreichii subsp. shermanii, under static flask conditions. Similarly, enhanced trehalose yield was reported in an osmotically sensitive mutant of the same strain under anaerobic conditions. In the present study, an effort was made to achieve higher production of trehalose, propionic acid, and lactic acid using the parent and an osmotically sensitive mutant of P. freudenreichii subsp. shermanii under aeration conditions. Under aeration conditions (200 rpm in shake flasks and 30 % air saturation in a batch reactor), biomass was increased and approximately 98 % of crude glycerol was consumed. In the parent strain, a trehalose titre of 361 mg/l was achieved, whereas in the mutant strain a trehalose titre of 1.3 g/l was produced in shake flask conditions (200 rpm). In the mutant strain, propionic and lactic acid yields of 0.53 and 0.21 g/g of substrate were also achieved with crude glycerol. Similarly, in controlled batch reactor culturing conditions a final trehalose titre of approximately 1.56 g/l was achieved with the mutant strain using crude glycerol as the substrate. Enhanced production of trehalose using P. freudenreichii subsp. shermanii from waste under aeration conditions is reported here. Higher production of trehalose was not due to a higher yield of trehalose but to a higher final biomass concentration. © 2012 Society for Industrial Microbiology and Biotechnology.
Citation: Journal of Industrial Microbiology and Biotechnology (2012), 39(8): 1153-1160
URI: https://doi.org/10.1007/s10295-012-1124-y
http://repository.iitr.ac.in/handle/123456789/1611
Issue Date: 2012
Keywords: Aeration
Biodiesel
Crude glycerol
Osmotically sensitive mutant
Propionibacterium freudenreichii
Trehalose
ISSN: 13675435
Author Scopus IDs: 54411237100
7103333522
Author Affiliations: Ruhal, R., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttrakhand 247667, India
Choudhury, B., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttrakhand 247667, India
Corresponding Author: Choudhury, B.; Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttrakhand 247667, India; email: bijanfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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