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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1607
Title: Alkaline pretreatment methods followed by acid hydrolysis of Saccharum spontaneum for bioethanol production
Authors: Chaudhary G.
Singh L.K.
Ghosh S.
Published in: Bioresource Technology
Abstract: Different alkaline pretreatment methods (NaOH, NaOH+10% urea and aqueous ammonia) were optimized for maximum delignification of Saccharum spontaneum at 30°C. Maximum delignification were obtained as 47.8%, 51% and 48% from NaOH (7% NaOH, 48h, and 10% biomass loading), NaOH+urea (7% NaOH+10% urea, 48h and 10% biomass loading) and 30% ammonia (40days and 10% biomass loading) respectively. H2SO4 60% (v/v), 10% biomass loading at 30°C for 4h, were optimized conditions to solubilize the cellulose and hemicellulose from solid residue obtained after different optimized alkaline pretreatments. Slurry thus obtained was diluted to obtain final acid concentration of 10% (v/v) for real hydrolysis of cellulose and hemicellulose at 100°C for 1h. Among all pretreatment methods applied, the best result 0.58g (85%) reducing sugars/g of initial biomass after acid hydrolysis was obtained from aqueous ammonia pretreated biomass. Scheffersomyces stipitis CBS6054 was used to ferment the hydrolysate; ethanol yield (Yp/s) and productivity (rp) were found to be 0.35g/g and 0.22g/L/h respectively. © 2012 Elsevier Ltd.
Citation: Bioresource Technology (2012), 124(): 111-118
URI: https://doi.org/10.1016/j.biortech.2012.08.067
http://repository.iitr.ac.in/handle/123456789/1607
Issue Date: 2012
Keywords: Acid hydrolysis
Alkaline pretreatment
Bioethanol
Bioprocess
Saccharum spontaneum (Kans Grass)
ISSN: 9608524
Author Scopus IDs: 57193339697
57206129327
56603000600
Author Affiliations: Chaudhary, G., Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India
Singh, L.K., Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India
Ghosh, S., Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India
Funding Details: Authors are thankful to University Grant Commission, New Delhi, India for providing financial assistance as Junior Research Fellowship (JRF) to Gaurav Chaudhary.
Corresponding Author: Ghosh, S.; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India; email: ghoshfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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