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Title: Bioconversion to chitosan: A two stage process employing chitin deacetylase from Penicillium oxalicum SAEM-51
Authors: Pareek N.
Vivekanand V.
Agarwal P.
Saroj S.
Singh, Rajesh Pratap
Published in: Carbohydrate Polymers
Abstract: Chitin deacetylase from Penicillium oxalicum SAEM-51 was evaluated for bioconversion of chitin to chitosan in a two stage chemical and enzymatic process. Variations in morphology, crystallinity and thermal properties following chemical treatment were evaluated by scanning electron microscopy, X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis. Degree of deacetylation of the substrates was determined using FT-IR and elemental analysis. The pretreatment of substrate led to the decrease in crystallinity and formation of amorphous chitinous substrates to facilitate enzyme reaction. The treated chitin was further subjected to enzymatic deacetylation employing chitin deacetylase from P. oxalicum SAEM-51 to produce chitosan with considerably higher degree of deacetylation. Maximum deacetylation (79.52%) was achieved using superfine chitin, owing to its porous structure and low crystallinity. Further, derivation of reaction variables, i.e. substrate amount and enzyme dose through full-factorial central composite design led to enhanced degree of deacetylation with formation of 90% deacetylated chitosan. © 2013 Elsevier Ltd. All rights reserved.
Citation: Carbohydrate Polymers(2013), 96(2): 417-425
Issue Date: 2013
Publisher: Elsevier Ltd
Keywords: Central composite design
Chitin deacetylase
Penicillium oxalicum
ISSN: 1448617
Author Scopus IDs: 35741588300
Author Affiliations: Pareek, N., Department of Chemistry, Umea University, SE-90183 Umea, Sweden
Vivekanand, V., Protein Engineering and Proteomics Group, Department of Chemistry, Biotechnology and Food Sciences, Norwegian University of Life Sciences, As 1430, Norway
Funding Details: NP gratefully acknowledges the fellowship support by Council of Scientific and Industrial Research, New Delhi, India .
Corresponding Author: Singh, R.P.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India; email:
Appears in Collections:Journal Publications [BT]

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