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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1581
Title: Synergistic action of cinnamaldehyde with silver nanoparticles against spore-forming bacteria: A case for judicious use of silver nanoparticles for antibacterial applications
Authors: Ghosh I.N.
Patil S.D.
Sharma T.K.
Srivastava S.K.
Pathania R.
Navani N.K.
Published in: International Journal of Nanomedicine
Abstract: Silver has long been advocated as an effective antimicrobial. However, toxicity issues with silver have led to limited use of silver in nanoform, especially for food preservation. With the aim of exploring combinatorial options that could increase the antibacterial potency of silver nanoparticles and reduce the effective dosage of silver, we evaluated the extent of synergy that a combination of silver nanoparticles and an essential oil representative (cinnamaldehyde) could offer. A battery of gram-positive and gram-negative bacterial strains was utilized for antibacterial assays, and extents of synergism were calculated from fractional inhibitory concentration indices. The activity of nanoparticles was greatly enhanced when utilized in the presence of cinnamaldehyde. We observed combinatorial effects that were strongly additive against all the bacterial strains tested, and genuine synergy was found against spore forming Bacillus cereus and Clostridium perfringens - bacterial strains associated with release of cytotoxins in contaminated food and known for their persistence. Bacterial kill curve analysis revealed a very fast bactericidal action when a combination of two agents was used. The electron and atomic force microscopy also revealed extensive damage to the bacterial cell envelop in the presence of both agents. We also performed hemolysis assays to investigate and approximate the extent of toxicity exhibited by the two agents, and observed no adverse effect at the concentrations required for synergy. This study shows that safe levels of silver in nanoform in combination with essential oil component cinnamaldehyde can be effectively used for controlling the spore-forming bacterial species. © 2013 Ghosh et al.
Citation: International Journal of Nanomedicine (2013), 8(): 4721-4731
URI: https://doi.org/10.2147/IJN.S49649
http://repository.iitr.ac.in/handle/123456789/1581
Issue Date: 2013
Publisher: Dove Medical Press Ltd.
Keywords: Antibacterial activity
Bacterial food spoilage
Erythrocyte toxicity
Essential oil
In vitro
Synergy
Toxin producing spore-forming bacteria
ISSN: 11769114
Author Scopus IDs: 57192421918
55614242900
56830083800
57202143131
7004308029
6507073924
Author Affiliations: Ghosh, I.N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Patil, S.D., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Sharma, T.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India, Center for Biodesign and Diagnostics, Translational Health Science and Technology Institute, Gurgaon, Haryana, India
Srivastava, S.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Pathania, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Navani, N.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Corresponding Author: Navani, N. K.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, District - Haridwar, Uttarakhand 247 667, India; email: navnifbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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