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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1580
Title: Methods of cell lysis and effect of detergents for the recovery of nitrile metabolizing enzyme from Amycolatopsis sp. IITR215
Authors: Babu V.
Choudhury B.
Published in: Journal of Genetic Engineering and Biotechnology
Abstract: Nitrile metabolizing enzymes are of great industrial interest for the selective bio-transformation of nitriles and surface modification of synthetic polymers under mild reaction conditions. In the present work, isolated strain Amycolatopsis sp. IITR215 was cultivated in the bench top bioreactor for the recovery of maximum biomass of whole cell catalyst. Effect of different lyoprotectants was studied on nitrile metabolizing enzyme from Amycolatopsis sp. IITR215 in which sorbitol proved to be an efficient lyoprotectant. In physical and mechanical methods, only 30% activity was recovered while 85% activity was achieved in the enzymatic method using 2 g/l lysozyme. Very less activity was recovered during stationary phase when cells were grown in mineral base media containing 1 g/l yeast. Therefore, recovery of intracellular enzymes was enhanced by using different concentrations of sodium cholate and deoxycholate. © 2013 .
Citation: Journal of Genetic Engineering and Biotechnology (2013), 11(2): 117-122
URI: https://doi.org/10.1016/j.jgeb.2013.05.002
http://repository.iitr.ac.in/handle/123456789/1580
Issue Date: 2013
Publisher: Academy of Scientific Research and Technology
Keywords: Amycolatopsis sp. IITR215
Cell lysis
Lysozyme
Nitrile metabolizing enzyme
ISSN: 1687157X
Author Scopus IDs: 8982627200
7103333522
Author Affiliations: Babu, V., Department of Biotechnology, Indian Institute of Technology, Roorkee, 247667, India, Department of Biotechnology, Graphic Era University, Dehradun, 248002, India
Choudhury, B., Department of Biotechnology, Indian Institute of Technology, Roorkee, 247667, India
Funding Details: The authors gratefully acknowledge the financial support from the Department of Biotechnology, Govt of India for providing the junior research fellowship to Vikash Babu.
Corresponding Author: Choudhury, B.; Department of Biotechnology, Indian Institute of TechnologyIndia; email: bijanfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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