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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1577
Title: Development and molecular characterization of wheat- Aegilops longissima derivatives with high grain micronutrients
Authors: Neelam K.
Rawat N.
Tiwari V.K.
Ghandhi N.
Arun P.C.
Kumar S.
Tripathi S.K.
Randhawa G.S.
Prasad R.
Dhaliwal H.S.
Published in: Australian Journal of Crop Science
Abstract: Developing food crops with enhanced mineral concentrations is one of the most sustainable and cost effective approaches for alleviation of micronutrient. This article aims at development and molecular characterization of wheat- Aegilops longissima derivatives with high grain micronutrients (iron, zinc, copper, manganese, calcium, magnesium and potassium). Aegilops longissima (2n=14, SlSl) accession 3506 with high grain micronutrients was used for transferring these traits to elite wheat (Triticum aestivum) cultivars through wide hybridization. The fertile HD2687/L3506//WL711 BC1F3 derivatives were developed through selfing and selection for chromosome constitution, meiotic stability and micronutrient concentrations was done at each generation. Sixteen derivatives were finally selected and characterized. The selected backcross derivatives showed enhanced grain iron, zinc, copper, manganese, calcium, magnesium and, potassium concentrations over the parental wheat cultivars by up to 183.6%, 243.6%, 135.18%, 160.42%, 223.29%, 43.90% and 35.05%, respectively. Introgression of chromosomes 2, 7 and 1 from Ae. longissima, confirmed by plant waxiness,GISH, anchored wheat SSR markers and HMW glutenin subunit profiling and was found to be associated with enhanced micronutrients in the derivatives.
Citation: Australian Journal of Crop Science (2013), 7(4): 508-514
URI: http://repository.iitr.ac.in/handle/123456789/1577
Issue Date: 2013
Keywords: Alien introgression
Biofortification
Grain iron
Grain zinc
Protein
ISSN: 18352693
Author Scopus IDs: 36904108200
24333068300
57196699090
55744015700
55744793600
8982913900
55735097900
7005416748
16690394400
35466824500
Author Affiliations: Neelam, K., School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
Rawat, N., Department of Plant Pathology, Kansas State University, Manhattan-66506, Kansas, United States
Tiwari, V.K., Department of Plant Pathology, Kansas State University, Manhattan-66506, Kansas, United States
Ghandhi, N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee-247667, Uttarakhand, India
Arun, P.C., Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, Pantnagar-249196, Uttarakhand, India
Kumar, S., Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, Pantnagar-249196, Uttarakhand, India
Tripathi, S.K., Department of Water Resources Development and Management, Indian Institute of Technology Roorkee, Roorkee-247667, Uttarakhand, India
Randhawa, G.S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee-247667, Uttarakhand, India
Prasad, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee-247667, Uttarakhand, India
Dhaliwal, H.S., Eternal University, Baru Sahib Via Rajgarh, Distt. Sirmour, Himachal Pradesh-173101, India
Corresponding Author: Tiwari, V.K.; Department of Plant Pathology, Kansas State University, Manhattan-66506, Kansas, United States; email: vijtiwari@ksu.edu
Appears in Collections:Journal Publications [BT]

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