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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1574
Title: Solvent free biocatalytic synthesis of isoniazid from isonicotinamide using whole cell of Bacillus smithii strain IITR6b2
Authors: Agarwal S.
Gupta M.
Choudhury B.
Published in: Journal of Molecular Catalysis B: Enzymatic
Abstract: A biocatalytic route for the synthesis of isoniazid, an important first-line antitubercular drug, in aqueous system is presented. The reported bioprocess is a greener method, does not involve any hazardous reagent and takes place under mild reaction conditions. Whole cell amidase of Bacillus smithii strain IITR6b2 having acyltransferase activity was utilized for its ability to transfer acyl group of isonicotinamide to hydrazine-2HCl in aqueous medium. B. smithii strain IITR6b2 possessed 3 folds higher acyltransferase activity as compared to amide hydrolase activity and this ratio was further improved to 4.5 by optimizing concentration of co-substrate hydrazine-2HCl. Various key parameters were optimized and under the optimum reaction conditions of pH (7, phosphate buffer 100 mM), temperature (30 C), substrate/co-substrate concentration (100/1000 mM) and resting cells concentration (2.0 mg dcw/ml), 90.4% conversion of isonicotinamide to isoniazid was achieved in 60 min. Under these conditions, a fed batch process for production of isoniazid was developed and resulted in the accumulation of 439 mM of isoniazid with 87.8% molar conversion yield and productivity of 6.0 g/h/g dcw. These results demonstrated that enzymatic synthesis of isoniazid using whole cells of B. smithii strain IITR6b2 might present an efficient alternative route to the chemical synthesis procedures without the involvement of organic solvent. © 2013 Elsevier B.V. All rights reserved.
Citation: Journal of Molecular Catalysis B: Enzymatic (2013), 97(): 67-73
URI: https://doi.org/10.1016/j.molcatb.2013.07.010
http://repository.iitr.ac.in/handle/123456789/1574
Issue Date: 2013
Keywords: Acyltransferase activity
Amidase
Bacillus smithii strain IITR6b2
Hydrazinolysis
Isoniazid
ISSN: 13811177
Author Scopus IDs: 57213773077
55766369700
7103333522
Author Affiliations: Agarwal, S., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India
Gupta, M., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India
Choudhury, B., Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India
Funding Details: The authors acknowledge the University Grant Commission, New Delhi for financial support in the form of Senior Research Fellowship to Ms. Shilpi agarwal.
Corresponding Author: Choudhury, B.; Bioprocess Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology, Roorkee 247667, Uttarakhand, India; email: bijanfbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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