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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1558
Title: Mustard oil cake as an inexpensive support for production of chitin deacetylase by Penicillium oxalicum SAEM-51 under solid-state fermentation
Authors: Pareek N.
Ghosh, Sanjoy
Singh, Rajesh Pratap
Vivekanand V.
Published in: Biocatalysis and Agricultural Biotechnology
Abstract: Response surface methodology was employed to optimize the cultivation conditions of Penicillium oxalicum SAEM-51 for the enhancement of chitin deacetylase (CDA) production under solid-state fermentation. The enzyme catalyzes deacetylation of N-acetyl glucosamine subunits of chitin resulted in the production of chitosan, widely utilized biopolymer for drug delivery, waste water treatment and as nutraceutics. Among different agro-horticultural substrates evaluated, mustard oil cake had resulted into maximal CDA production. Entrapment of fungal mycelia onto the solid support was studied using scanning electron microscopy. Optimal physico-chemical conditions for maximal CDA production were found to be 4.906 g, 73.62% and 8.578% for substrate amount, moisture content and inoculum size, respectively. The experimental CDA production (1162.03 ± 7.2 U gds-1) under optimized condition was observed in close agreement with the values predicted by the quadratic model (1137.85Ugds-1). The CDA production by P. oxalicum SAEM-51 was increased significantly by 1.3 fold, as compared to the un-optimized ones (877.56 ± 8.9Ugds-1). © 2014 Elsevier Ltd.
Citation: Biocatalysis and Agricultural Biotechnology (2014), 3(4): 212-217
URI: https://doi.org/10.1016/j.bcab.2014.04.002
http://repository.iitr.ac.in/handle/123456789/1558
Issue Date: 2014
Publisher: Elsevier Ltd
Keywords: Central composite design
Chitin deacetylase
Penicillium oxalicum
Solid state fermentation
ISSN: 18788181
Author Scopus IDs: 35741588300
56603000600
57208931324
57211114758
Author Affiliations: Pareek, N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India, Department of Microbiology, School of Life Sciences, Central University of Rajasthan, Bandarsindri, Kishangarh, Ajmer, Rajasthan, 305801, India
Ghosh, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India
Singh, R.P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India
Vivekanand, V., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247 667, India, Protein Engineering and Proteomics Group, Departmentof Chemistry, Biotechnology and Food Sciences, Norwegian University of Life Sciences, Âs, 1430, Norway
Funding Details: NP gratefully acknowledges the fellowship support by Council of Scientific and Industrial Research, New Delhi, India .
Corresponding Author: Pareek, N.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia
Appears in Collections:Journal Publications [BT]

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