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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1554
Title: Purification and physicochemical characterization of a trypsin inhibitor from cassia absus linn
Authors: Patel G.K.
Gupta A.K.
Gupta A.
Mishra M.
Singh P.K.
Saxena A.K.
Sharma A.K.
Published in: Protein and Peptide Letters
Abstract: A thermotolerant protein with trypsin inhibitory activity designated as CaTI was purified to homogeneity from seeds of Cassia absus. Gel filtration chromatography and SDS-PAGE analysis showed the apparent molecular mass of ?20 kDa. Partial internal sequences indicate that CaTI belongs to Kunitz-inhibitor family. CaTI inhibits the bovine trypsin in 1:1 molar ratio and exhibited a competitive-type inhibitory activity with Ki = 5.6 × 10-9 M. The inhibitory activity was retained over a broad pH range (2-12). Thermal stability study showed that it is stable up to 80 °C and inhibition activity reduced at and above 90 °C which might be due to the presence of predominantly ?-sheets revealed by the CD study. The proteolysis studies of CaTI exhibited strong resistance to proteolysis by different proteases tested. The studies show that CaTI can be used as potential candidates for the development of the transgenic plant against the microbes and insect pests. © 2014 Bentham Science Publishers.
Citation: Protein and Peptide Letters(2014), 21(2): 108-114
URI: https://doi.org/10.2174/09298665113209990090
http://repository.iitr.ac.in/handle/123456789/1554
Issue Date: 2014
Publisher: Bentham Science Publishers
Keywords: Caesalpiniaceae
Cassia absus
Kinetic study
Stability studies
Trypsin inhibitor
ISSN: 9298665
Author Scopus IDs: 57192590014
57205674292
55628527188
56357223700
55252883200
7401482532
57214355701
Author Affiliations: Patel, G.K., Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India, Department of Biotechnology, Graphic Era University, Dehradun-248002, India
Gupta, A.K., Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow-226001, India
Gupta, A., Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India
Mishra, M., Plant Molecular Biology Division, CSIRNational Botanical Research Institute, Lucknow-226001, India
Singh, P.K., Plant Molecular Biology Division, CSIRNational Botanical Research Institute, Lucknow-226001, India
Saxena, A.K., Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow-226001, India
Sharma, A.K., Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India
Corresponding Author: Sharma, A.K.; Department of Biotechnology, Indian Institute of Technology, Roorkee-247667, India; email: aksbsfbs@yahoo.co.in
Appears in Collections:Journal Publications [BT]

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