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Title: Boosting accumulation of neutral lipids in Rhodosporidium kratochvilovae HIMPA1 grown on hemp (Cannabis sativa Linn) seed aqueous extract as feedstock for biodiesel production
Authors: Patel A.
Pravez M.
Deeba F.
Pruthi V.
Singh, Rajesh Pratap
Pruthi P.A.
Published in: Bioresource Technology
Abstract: Hemp seeds aqueous extract (HSAE) was used as cheap renewable feedstocks to grow novel oleaginous yeast Rhodosporidium kratochvilovae HIMPA1 isolated from Himalayan permafrost soil. The yeast showed boosted triglyceride (TAG) accumulation in the lipid droplets (LDs) which were transesterified to biodiesel. The sonicated HSAE prepared lacked toxic inhibitors and showed enhanced total lipid content and lipid yield 55.56%, 8.39±0.57g/l in comparison to 41.92%, 6.2±0.8g/l from industrially used glucose synthetic medium, respectively. Supersized LDs (5.95±1.02?m) accumulated maximum TAG in sonicated HSAE grown cells were visualized by fluorescent BODIPY (505/515nm) stain. GC-MS analysis revealed unique longer carbon chain FAME profile containing Arachidic acid (C20:0) 5%, Behenic acid (C22:0) 9.7%, Heptacosanoic acid (C27:0) 14.98%, for the first time in this yeast when grown on industrially competent sonicated HSAE, showing more similarity to algal oils. © 2014.
Citation: Bioresource Technology(2014), 165(C): 214-222
Issue Date: 2014
Publisher: Elsevier Ltd
Keywords: Biodiesel
Rhodosporidium kratochvilovae HIMPA1
Sonicated hemp seed aqueous extract
ISSN: 9608524
Author Scopus IDs: 55930061600
Author Affiliations: Patel, A., Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand, 247667, India
Pravez, M., Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Tech
Funding Details: Authors are thankful for financial support by the Department of Biotechnology, Govt. of India , BioCare Programme, DBT Sanction No.: 102/IFD/SAN/3539/2011-2012 (Grant No.: DBT-608-BIO ) and JRF fellowship to AKP from University Grant Commission , India (G
Corresponding Author: Pruthi, P.A.; Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R)India
Appears in Collections:Journal Publications [BT]

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