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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1549
Title: Genetic regulation of spy gene expression in Escherichia coli in the presence of protein unfolding agent ethanol
Authors: Srivastava S.K.
Lambadi P.R.
Ghosh T.
Pathania, R.
Navani, Naveen Kumar
Published in: Gene
Abstract: In a living cell, folding of proteins is assisted by molecular chaperones and other folding helpers. In Escherichia coli (E. coli), recently an ATP independent chaperon 'Spy' was discovered which is highly up-regulated in the presence of protein unfolding agents like ethanol, butanol and tannic acid. Two response regulators; BaeR and CpxR have been recognized as transcriptional regulators of spy gene. However, the mechanism of genetic regulation of spy under protein denaturants like ethanol has not been studied in detail so far. Based on a combination of genetic, molecular biology and biochemical experimental data, we propose that BaeR protein is the primary regulator of spy gene in response to ethanol stress in E. coli. In addition, we expanded the experimental spectrum and validated that regulation of spy gene in the presence of zinc and copper metal stress is primarily via BaeR and CpxR regulators respectively. We also performed in-silico analysis to identify the homologs of Spy protein and their cognate regulatory elements in bacterial species belonging to enterobacteriaceae family. Based on the unique ATP-independent chaperone nature and genetic regulation of spy we also propose its importance in biosensor development and facilitated production of properly folded recombinant proteins. © 2014 Elsevier B.V.
Citation: Gene (2014), 548(1): 142-148
URI: https://doi.org/10.1016/j.gene.2014.07.003
http://repository.iitr.ac.in/handle/123456789/1549
Issue Date: 2014
Publisher: Elsevier
Keywords: BaeR regulator
Chaperones
CpxR regulator
Ethanol stress
Spy
ISSN: 3781119
Author Scopus IDs: 57202143131
56254414300
57197638847
7004308029
6507073924
Author Affiliations: Srivastava, S.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Uttarakhand 247 667, India
Lambadi, P.R., Department of Biotechnology, Indian Institute of Technology Roorkee, Uttarakhand 247 667, India
Ghosh, T., Department of Biotechnology, Indian Institute of Technology Roorkee, Uttarakhand 247 667, India
Pathania, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Uttarakhand 247 667, India
Navani, N.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Uttarakhand 247 667, India
Funding Details: This work is supported by National Agriculture Innovation Project (NAIP) grant number C4-30032 of the Indian Council of Agricultural Research (ICAR) and DBT grant number BT-MED-TF(3) 2012 Govt. of India to NKN and RP respectively. We thank Professor Steven Lindow at the University of California, Berkeley USA for a kind gift of plasmid pPROBE-TT?-GFP.
Corresponding Author: Navani, N.K.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247 667, India; email: navnifbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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