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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1540
Title: Characterization of anticancer, DNase and antifungal activity of pumpkin 2S albumin
Authors: Tomar P.P.S.
Nikhil K.
Singh A.
Selvakumar P.
Roy, Partha
Sharma, Ashwani Kumar
Published in: Biochemical and Biophysical Research Communications
Abstract: The plant 2S albumins exhibit a spectrum of biotechnologically exploitable functions. Among them, pumpkin 2S albumin has been shown to possess RNase and cell-free translational inhibitory activities. The present study investigated the anticancer, DNase and antifungal activities of pumpkin 2S albumin. The protein exhibited a strong anticancer activity toward breast cancer (MCF-7), ovarian teratocarcinoma (PA-1), prostate cancer (PC-3 and DU-145) and hepatocellular carcinoma (HepG2) cell lines. Acridine orange staining and DNA fragmentation studies indicated that cytotoxic effect of pumpkin 2S albumin is mediated through induction of apoptosis. Pumpkin 2S albumin showed DNase activity against both supercoiled and linear DNA and exerted antifungal activity against Fusarium oxysporum. Secondary structure analysis by CD showed that protein is highly stable up to 90 °C and retains its alpha helical structure. These results demonstrated that pumpkin 2S albumin is a multifunctional protein with host of potential biotechnology applications. © 2014 Elsevier Inc. All rights reserved.
Citation: Biochemical and Biophysical Research Communications(2014), 448(4): 349-354
URI: https://doi.org/10.1016/j.bbrc.2014.04.158
http://repository.iitr.ac.in/handle/123456789/1540
Issue Date: 2014
Publisher: Academic Press Inc.
Keywords: Anticancer activity
Antifungal activity
CD studies
Deoxyribonuclease activity
Pumpkin 2S albumin
ISSN: 0006291X
Author Scopus IDs: 54906718800
57192281884
56130503700
36493749300
35509207200
57214355701
Author Affiliations: Tomar, P.P.S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India
Nikhil, K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India
Singh, A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India
Selvakumar, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India
Roy, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India
Sharma, A.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India
Funding Details: This work was supported by a Grant No. IRIS ID No. 2011-15280 from Indian Council of Medical Research , Government of India. CD studies and gel-exclusion chromatography were performed at NMR facility and Macromolecular Crystallographic Unit respectively at Institute Instrumentation Centre (IIC), IIT Roorkee. P.P.S.T. and K.N. thanks CSIR , P.S. and A.S. thanks DBT , Government of India for financial support. Appendix A
Corresponding Author: Sharma, A.K.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India; email: aksbsfbs@yahoo.co.in
Appears in Collections:Journal Publications [BT]

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