http://repository.iitr.ac.in/handle/123456789/1540| Title: | Characterization of anticancer, DNase and antifungal activity of pumpkin 2S albumin |
| Authors: | Tomar P.P.S. Nikhil K. Singh A. Selvakumar P. Roy, Partha Sharma, Ashwani Kumar |
| Published in: | Biochemical and Biophysical Research Communications |
| Abstract: | The plant 2S albumins exhibit a spectrum of biotechnologically exploitable functions. Among them, pumpkin 2S albumin has been shown to possess RNase and cell-free translational inhibitory activities. The present study investigated the anticancer, DNase and antifungal activities of pumpkin 2S albumin. The protein exhibited a strong anticancer activity toward breast cancer (MCF-7), ovarian teratocarcinoma (PA-1), prostate cancer (PC-3 and DU-145) and hepatocellular carcinoma (HepG2) cell lines. Acridine orange staining and DNA fragmentation studies indicated that cytotoxic effect of pumpkin 2S albumin is mediated through induction of apoptosis. Pumpkin 2S albumin showed DNase activity against both supercoiled and linear DNA and exerted antifungal activity against Fusarium oxysporum. Secondary structure analysis by CD showed that protein is highly stable up to 90 °C and retains its alpha helical structure. These results demonstrated that pumpkin 2S albumin is a multifunctional protein with host of potential biotechnology applications. © 2014 Elsevier Inc. All rights reserved. |
| Citation: | Biochemical and Biophysical Research Communications(2014), 448(4): 349-354 |
| URI: | https://doi.org/10.1016/j.bbrc.2014.04.158 http://repository.iitr.ac.in/handle/123456789/1540 |
| Issue Date: | 2014 |
| Publisher: | Academic Press Inc. |
| Keywords: | Anticancer activity Antifungal activity CD studies Deoxyribonuclease activity Pumpkin 2S albumin |
| ISSN: | 0006291X |
| Author Scopus IDs: | 54906718800 57192281884 56130503700 36493749300 35509207200 57214355701 |
| Author Affiliations: | Tomar, P.P.S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India Nikhil, K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India Singh, A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India Selvakumar, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India Roy, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India Sharma, A.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India |
| Funding Details: | This work was supported by a Grant No. IRIS ID No. 2011-15280 from Indian Council of Medical Research , Government of India. CD studies and gel-exclusion chromatography were performed at NMR facility and Macromolecular Crystallographic Unit respectively at Institute Instrumentation Centre (IIC), IIT Roorkee. P.P.S.T. and K.N. thanks CSIR , P.S. and A.S. thanks DBT , Government of India for financial support. Appendix A |
| Corresponding Author: | Sharma, A.K.; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India; email: aksbsfbs@yahoo.co.in |
| Appears in Collections: | Journal Publications [BT] |
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