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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1524
Title: Synergistic effect of fermentable and non-fermentable carbon sources enhances TAG accumulation in oleaginous yeast Rhodosporidium kratochvilovae HIMPA1
Authors: Patel A.
Pruthi V.
Singh, Rajesh Pratap
Pruthi P.A.
Published in: Bioresource Technology
Abstract: Novel strategy for enhancing TAG accumulation by simultaneous utilization of fermentable and non-fermentable carbon sources as substrate for cultivation of oleaginous yeast Rhodosporidium kratochvilovae HIMPA1 were undertaken in this investigation. The yeast strain showed direct correlation between the size of lipid bodies, visualized by BODIPY stain (493-515nm) and TAG accumulation when examined on individual fermenting and non-fermenting carbon sources and their mixtures. Maximum TAG accumulation (?m) in glucose (2.38±0.52), fructose (4.03±0.38), sucrose (4.24±0.45), glycerol (4.35±0.54), xylulose (3.94±0.12), and arabinose (2.98±0.43) were observed. Synergistic effect of the above carbon sources (fermentable and non-fermentable) in equimolar concentration revealed maximum lipid droplet size of 5.35±0.76?m and cell size of 6.89±0.97?m. Total lipid content observed in mixed carbon sources was 9.26g/l compared to glucose (6.2g/l). FAME profile revealed enhanced longer chain (C14:0-C24:0) fatty acids in mix carbon sources. © 2015 Elsevier Ltd.
Citation: Bioresource Technology(2015), 188(): 136-144
URI: https://doi.org/10.1039/c6ra05266e
http://repository.iitr.ac.in/handle/123456789/1524
Issue Date: 2015
Publisher: Elsevier Ltd
Keywords: Fermenting
Nonfermenting
Oleaginous
TAG
Yeast
ISSN: 9608524
Author Scopus IDs: 55930061600
18837702800
57208931324
25958285300
Author Affiliations: Patel, A., Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand, 247667, India
Pruthi, V., Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Tech
Funding Details: Authors are thankful for financial support by the Department of Biotechnology, Govt. of India, BioCare Programme , DBT Sanction No. 102/IFD/SAN/3539/2011-2012 (Grant No. DBT-608-BIO ) and JRF fellowship to Alok Kumar Patel from University Grant Commission
Corresponding Author: Pruthi, P.A.; Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT R)India
Appears in Collections:Journal Publications [BT]

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