Skip navigation
Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1517
Title: Isolation and characterization of a protease-producing novel haloalkaliphilic bacterium Halobiforma sp. strain BNMIITR from Sambhar lake in Rajasthan, India
Authors: Gupta M.
Aggarwal S.
Navani, Naveen Kumar
Choudhury, Bijan
Published in: Annals of Microbiology
Abstract: A novel haloalkaliphilic bacterium designated as strain BNMIITR was isolated from a soil sample collected from Sambhar lake, Rajasthan, in northern India. Colonies of the isolated strain were dark orange and comprised Gram-negative bacilli; there was a slight pleomorphism towards the stationary phase of growth. Experiments revealed that the isolate can grow in the range of 2–5 M NaCl, pH 6–11 and 18–55 °C, with optimum growth observed at 3 M NaCl, pH 8–8.5 and 45 °C. No growth was observed in culture medium without NaCl. The isolate showed no requirement for magnesium sulphate heptahydrate (MgSO4 .7H2O) for growth. Major cellular fatty acids were C 14:0, C 15:0 iso, C 15:0 anteiso, C 16:0, C 17:0 iso, C 17:0 anteiso and C 20:2 w6, 9c. The result of 16S rRNA gene sequence analysis showed 98 % sequence similarity with Halobiforma lacisalsi and Hbf. haloterrestris. Halobiforma sp. strain BNMIITR showed resistance towards several antibiotics and produced an extracellular alkaline protease. The crude enzyme was found to be active in broad range of alkaline pH and temperature (30–80 °C). © 2014, Springer-Verlag Berlin Heidelberg and the University of Milan.
Citation: Annals of Microbiology (2015), 65(2): 677-686
URI: https://doi.org/10.1007/s13213-014-0906-z
http://repository.iitr.ac.in/handle/123456789/1517
Issue Date: 2015
Publisher: Springer Verlag
Keywords: Cellular fatty acids
Haloalkaliphilic
Halobiforma
Pleomorphism
Protease
ISSN: 15904261
Author Scopus IDs: 55766369700
57197912066
6507073924
7103333522
Author Affiliations: Gupta, M., Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247667, India
Aggarwal, S., Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247667, India
Navani, N.K., Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247667, India
Choudhury, B., Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee, Roorkee, Uttarakhand 247667, India
Corresponding Author: Choudhury, B.; Department of Biotechnology, Indian Institute of Technology (IIT) RoorkeeIndia
Appears in Collections:Journal Publications [BT]

Files in This Item:
There are no files associated with this item.
Show full item record


Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.