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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1514
Title: Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control
Authors: Lambadi P.R.
Sharma T.K.
Kumar P.
Vasnani P.
Thalluri S.M.
Bisht N.
Pathania, R.
Navani, Naveen Kumar
Published in: International Journal of Nanomedicine
Abstract: Infectious diseases cause a huge burden on healthcare systems worldwide. Pathogenic bacteria establish infection by developing antibiotic resistance and modulating the host’s immune system, whereas opportunistic pathogens like Pseudomonas aeruginosa adapt to adverse conditions owing to their ability to form biofilms. In the present study, silver nanoparticles were biofunctionalized with polymyxin B, an antibacterial peptide using a facile method. The biofunctionalized nanoparticles (polymyxin B-capped silver nanoparticles, PBSNPs) were assessed for antibacterial activity against multiple drug-resistant clinical strain Vibrio fluvialis and nosocomial pathogen P. aeruginosa. The results of antibacterial assay revealed that PBSNPs had an approximately 3-fold higher effect than the citrate-capped nanoparticles (CSNPs). Morphological damage to the cell membrane was followed by scanning electron microscopy, testifying PBSNPs to be more potent in controlling the bacterial growth as compared with CSNPs. The bactericidal effect of PBSNPs was further confirmed by Live/Dead staining assays. Apart from the antibacterial activity, the biofunctionalized nanoparticles were found to resist biofilm formation. Electroplating of PBSNPs onto stainless steel surgical blades retained the antibacterial activity against P. aeruginosa. Further, the affinity of polymyxin for endotoxin was exploited for its removal using PBSNPs. It was found that the prepared nanoparticles removed 97% of the endotoxin from the solution. Such multifarious uses of metal nanoparticles are an attractive means of enhancing the potency of antimicrobial agents to control infections. © 2015 Lambadi et al.
Citation: International Journal of Nanomedicine (2015), 10(): 2155-2171
URI: https://doi.org/10.2147/IJN.S72923
http://repository.iitr.ac.in/handle/123456789/1514
Issue Date: 2015
Publisher: Dove Medical Press Ltd.
Keywords: Biofilm inhibition
Biofunctionalization
Endotoxin removal
Nosocomial pathogen
Polymyxin B
Silver nanoparticles
ISSN: 11769114
Author Scopus IDs: 56254414300
56830083800
57216399468
56600860900
55965214900
56600258900
7004308029
6507073924
Author Affiliations: Lambadi, P.R., Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Sharma, T.K., Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Kumar, P., Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Vasnani, P., Centre of Nanotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Thalluri, S.M., Centre of Nanotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Bisht, N., Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Pathania, R., Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India, Centre of Nanotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Navani, N.K., Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India, Centre of Nanotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India
Corresponding Author: Navani, N.K.; Chemical Biology Laboratory, Department of Biotechnology and Centre of Nanotechnology, Indian Institute of TechnologyIndia; email: navnifbs@iitr.ernet.in
Appears in Collections:Journal Publications [BT]

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