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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1504
Title: Antibacterial potential of a small peptide from Bacillus sp. RPT-0001 and its capping for green synthesis of silver nanoparticles
Authors: Patil S.D.
Sharma R.
Bhattacharyya T.
Kumar P.
Gupta M.
Chaddha B.S.
Navani N.K.
Pathania R.
Published in: Journal of Microbiology
Abstract: Infirmity and death from diseases caused by unsafe food are a continual hazard to communal health safety and socio-economic growth throughout the world. Chemical preservatives are associated with health hazards and toxicity issues. In the study reported here, 200 soil isolates from Western Himalayan region in India were screened for potential antibacterial activity against food-borne pathogens. This study led to the isolation of a bacterial strain belonging to the Genus Bacillus and was designated as RPT-0001. The associated antibacterial activity was sensitive to pronase E treatment. Bioassay-guided fractionation using reverse phase high performance liquid chromatography (RP-HPLC) led to isolation of the antibacterial peptide designated as RPT-0001. The molecular weight of RPT-0001 was determined by electro-spray ionization mass spectroscopy (ESI-MS) as 276.9 Da. RPT-0001 was inhibitory to both Gram-negative and Grampositive food-borne bacteria tested. The characteristics of RPT-0001 do not match with that of any other known antibacterial peptides produced by Bacillus sp. or related genera. Purified RPT-0001 was successfully used in synthesis of silver nanoparticles effective against food-borne pathogenic bacteria. The antibacterial peptide and silver nanoparticles synthesized utilizing it as a capping and reducing agent hold promising potential in food preservation, in packaging material and as a therapeutic agent in the treatment of foodborne infections. © 2015, The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.
Citation: Journal of Microbiology (2015), 53(9): 643-652
URI: https://doi.org/10.1007/s12275-015-4686-3
http://repository.iitr.ac.in/handle/123456789/1504
Issue Date: 2015
Publisher: Microbiological Society of Korea
Keywords: antibacterial
antibacterial peptide
ESI-MS
food-borne pathogens
RP-HPLC
silver nanoparticles
soil bacteria
ISSN: 12258873
Author Scopus IDs: 55614242900
57212897407
56763662400
57216399468
56763211000
57205003149
6507073924
7004308029
Author Affiliations: Patil, S.D., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Sharma, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Bhattacharyya, T., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Kumar, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Gupta, M., Centre for Excellence in disaster Mitigation and Management, Indian Institute of Technology Roorkee, Roorkee, India
Chaddha, B.S., Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, India
Navani, N.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Pathania, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Corresponding Author: Pathania, R.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: ranjanapathania@gmail.com
Appears in Collections:Journal Publications [BT]

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