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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1493
Title: Characterization and cloning of an 11S globulin with hemagglutination activity from Murraya paniculata
Authors: Singh A.
Selvakumar P.
Saraswat A.
Tomar P.P.S.
Mishra M.
Singh P.K.
Sharma, Ashwani Kumar
Published in: Protein and Peptide Letters
Abstract: A ?56 kDa protein having hemagglutination activity was purified and characterized from the Murraya paniculata seeds. The gel electrophoresis studies demonstrated that protein is primarily of two different subunits, molecular weight ? 35 and 21 kDa held together by disulfide-linkages and predominantly by secondary forces. The cloning and sequence analysis revealed that the protein exhibited a substantial sequence identity to seed storage 11S globulin family proteins. The sequence analysis of Murraya paniculata globulin (MPG) demonstrated higher and lower molecular weight polypeptides to be acidic (?) and basic (?) respectively. The sequence analysis further showed that it possesses a characteristic bi-cupin motif and a putative metal binding pocket. CD analysis revealed that the MPG was a ?/? protein with a slightly higher content of the former. Conformational changes in protein have been studied by fluorescence spectrometry by using various chemical treatments. The results demonstrated that MPG belongs to 11S globulin family and exhibit's hemagglutination activity, which implicates it to be possessing lectin-like property. © 2015 Bentham Science Publishers.
Citation: Protein and Peptide Letters(2015), 22(8): 750-761
URI: https://doi.org/10.2174/0929866522666150529161704
http://repository.iitr.ac.in/handle/123456789/1493
Issue Date: 2015
Publisher: Bentham Science Publishers B.V.
Keywords: 11S globulin
Cupin motif
Hemagglutination activity
Metal binding
Murraya paniculata
ISSN: 9298665
Author Scopus IDs: 56130503700
36493749300
54684995600
54906718800
56357223700
55252883200
57214355701
Author Affiliations: Singh, A., Protein Biochemistry and Crystallography Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India
Selvakumar, P., Protein Biochemistry and Crystallography Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India
Saraswat, A., Protein Biochemistry and Crystallography Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India
Tomar, P.P.S., Protein Biochemistry and Crystallography Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India
Mishra, M., Plant Molecular Biology Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, India, Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, 2-Rafi Marg, New Delhi, India
Singh, P.K., Protein Biochemistry and Crystallography Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India
Sharma, A.K., Protein Biochemistry and Crystallography Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247 667, India
Corresponding Author: Sharma, A.K.; Protein Biochemistry and Crystallography Laboratory, Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: aksbsfbs@yahoo.co.in
Appears in Collections:Journal Publications [BT]

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