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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/14885
Title: Surface modified goat-lung decellularized scaffold for bio-artificial skin tissue engineering applications
Authors: Gupta S.K.
Potdar P.D.
Dinda A.K.
Mishra, Narayan Chandra
Published in: Journal of Biomaterials and Tissue Engineering
Abstract: In the present study, goat-lung was decellularized and used as a scaffold to support cellular attachment and proliferation. The biological properties of decellularized goat-lung scaffold were enhanced by modifying it with collagen and gelatin, and the effect of modification was evaluated on the growth of human skin-derived mesenchymal stem cells (S-MSCs). MTT assay demonstrated good biocompatibility, cell adhesion and proliferation of S-MSCs over collagen and gelatin modified scaffolds as compared to unmodified decellularized scaffold. Scanning electron microscopy observation confirmed the distribution of S-MSCs in the gelatin and collagen modified decellularized scaffold. Hematoxylin and Eosin staining further revealed that collagen and gelatin modified decellularized scaffold maintained good biocompatibility with S-MSCs. Expression of Keratin18 gene, along with CD105, CD73 and CD44, by S-MSCs over collagen and gelatin modified decellularized scaffold signifies that the cells are viable, proliferating and migrating, and have maintained their critical cellular functions within the scaffold. All the above results suggests that modification of goat-lung decellularized scaffold by collagen and gelatin enhances the growth and proliferation of S-MSCs, and therefore, collagen and gelatin modified decellularized scaffold could be a potential candidate for bio-artificial skin tissue engineering applications. © 2013 American Scientific Publishers, All rights reserved.
Citation: Journal of Biomaterials and Tissue Engineering (2013), 3(6): 643-652
URI: https://doi.org/10.1166/jbt.2013.1122
http://repository.iitr.ac.in/handle/123456789/14885
Issue Date: 2013
Publisher: American Scientific Publishers
Keywords: Goat-lung
Scaffold
Skin
Stem cells
Tissue engineering
ISSN: 21579083
Author Scopus IDs: 56968266700
6602889612
7005837526
36798999600
Author Affiliations: Gupta, S.K., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, UP 247001, India
Potdar, P.D., Department of Molecular Medicine and Biology, Jaslok Hospital and Research Center, Mumbai, Maharashtra 400026, India
Dinda, A.K., Department of Pathology, All India Institute of Medical Sciences, New Delhi 110029, India
Mishra, N.C., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, UP 247001, India
Corresponding Author: Mishra, N. C.; Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, UP 247001, India
Appears in Collections:Journal Publications [PE]

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