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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1482
Title: Assessment of estradiol-induced gene regulation and proliferation in an immortalized mouse immature Sertoli cell line
Authors: Kumar N.
Srivastava S.
Burek M.
Förster C.Y.
Roy P.
Published in: Life Sciences
Abstract: Aims The number of Sertoli cells during proliferative phase determines the fate of the germ cells in male reproductive system. A well-characterized cell line may help in better understanding of Sertoli cell biology. Hence, the present study assessed estradiol signaling in a mouse immature Sertoli cell line (MSC-1) as an alternative model in place of primary culture of Sertoli cells. Main methods In this study, we used MSC-1 cell line, derived from 10-day old mice. The cell cycle parameters were assessed, and the expression and regulation of Sertoli cell-specific secretory genes (ABP; androgen-binding protein) and tight junction genes (claudin-5, occludin, and vimentin) in response to estradiol was studied. Key findings The results obtained suggested the presence of both estrogen receptors (ER? and ER?) in MSC-1 cells. In vitro scratch assay and cell-cycle analysis suggested the proliferative effects of estradiol in both time- and dose-dependent manner. The gene expression profiles of ABP, claudin-5, and occludin showed biphasic regulation at low and high doses of estradiol. Analysis of signaling pathways suggested the activation of extracellular signal-regulated kinase (ERK) pathway with significantly increased pERK/ERK ratio (p < 0.05). The results also suggested down regulation in the expression of mir-17 family members (mir-17, mir-20b, and mir-106a) (p < 0.05). Significance Considering the limited number of Sertoli cell lines and long-term survival inability of primary culture of Sertoli cells, MSC-1 cells could be a potential cell line for understanding the mechanisms of various cellular events in Sertoli cells. © 2016 Elsevier Inc. All rights reserved.
Citation: Life Sciences (2016), 148(): 268-278
URI: https://doi.org/10.1016/j.lfs.2016.01.027
http://repository.iitr.ac.in/handle/123456789/1482
Issue Date: 2016
Publisher: Elsevier Inc.
Keywords: Epigenetic
Estrogen receptors
Estrogens
MAPK pathway
MicroRNAs
Sertoli cell proliferation
ISSN: 243205
Author Scopus IDs: 57211774725
40262604000
56593341900
7201641459
35509207200
Author Affiliations: Kumar, N., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247 667, India
Srivastava, S., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247 667, India
Burek, M., University Wurzburg, Department of Anaesthesia and Critical Care, Oberduerrbacher Strasse 6, Wurzburg, 97080, Germany
Förster, C.Y., University Wurzburg, Department of Anaesthesia and Critical Care, Oberduerrbacher Strasse 6, Wurzburg, 97080, Germany
Roy, P., Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247 667, India
Funding Details: This work was supported by the Department of Biotechnology (No. BT/PR 14836/AAQ/01/455/2010 ) and Council of Scientific and Industrial Research (No. 37(1402)/10/EMR II ), Government of India as funded projects to PR. NK thanks the Department of Biotechnology, Government of India for fellowship and research support (Reference no. DBT-JRF/2009-10/481 ).
Corresponding Author: Roy, P.; Molecular Endocrinology Laboratory, Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: paroyfbs@iitr.ac.in
Appears in Collections:Journal Publications [BT]

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