http://repository.iitr.ac.in/handle/123456789/1481
Title: | Drug functionalized microbial polysaccharide based nanofibers as transdermal substitute |
Authors: | Vashisth P. Srivastava A.K. Nagar H. Raghuwanshi N. Sharan S. Nikhil K. Pruthi P.A. Singh, Rajesh Pratap Roy, Partha Pruthi V. |
Published in: | Nanomedicine: Nanotechnology, Biology, and Medicine |
Abstract: | In order to promote the natural healing process, drug-functionalized nanofibrous transdermal substitute was fabricated using gellan as chief polymer and polyvinyl alcohol (PVA) as supporting polymer via electrospinning technique. These fabricated nanofibers physiochemically mimic the extracellular matrix (ECM) which supports the cell growth. For neo-tissue regeneration in a sterilized environment, amoxicillin (Amx) was entrapped within these nanofibers. Entrapment of Amx in the nanofibers was confirmed by FESEM, FTIR, XRD and TG analysis. In vitro cell culture studies revealed that the fabricated non-cytotoxic nanofibers promoted enhance cell adherence and proliferation of human keratinocytes. A preliminary in vivo study performed on rat model for full thickness skin excision wound demonstrated the prompt re-epithelialization in early phase and quicker collagen deposition in later phases of wound healing in case of Amx-functionalized gellan/PVA nanofibers. Data collectively confirmed the potential usage of gellan based electrospun nanofibers as transdermal substitute for faster skin restoration. © 2015 Elsevier Inc. |
Citation: | Nanomedicine: Nanotechnology, Biology, and Medicine(2016), 12(5): 1375-1385 |
URI: | https://doi.org/10.1016/j.bbagen.2016.11.011 http://repository.iitr.ac.in/handle/123456789/1481 |
Issue Date: | 2016 |
Publisher: | Elsevier Inc. |
Keywords: | Electrospinning Gellan Nanofibers Transdermal substitute Wound healing |
ISSN: | 15499634 |
Author Scopus IDs: | 55930984400 57188662614 55421031200 57188664595 55661549600 57188662650 25958285300 57208931324 35509207200 18837702800 |
Author Affiliations: | Vashisth, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India Srivastava, A.K., Sapience Bio-Analytical Research Lab Bhopal, Madhya Pradesh, India Nagar, H., Sapience Bio-Analytical Research Lab Bhopal, |
Funding Details: | Authors are also thankful to Dr. Narayan C. Mishra, and Institute Instrumentation Center, Indian Institute of Technology, Roorkee for providing research facilities for this work. |
Corresponding Author: | Pruthi, V.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: vikasfbs@iitr.ernet.in |
Appears in Collections: | Journal Publications [BT] |
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