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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/14789
Title: Modification of decellularized goat-lung scaffold with chitosan/ nanohydroxyapatite composite for bone tissue engineering applications
Authors: Gupta S.K.
Dinda A.K.
Potdar P.D.
Mishra, Narayan Chandra
Published in: BioMed Research International
Abstract: Decellularized goat-lung scaffold was fabricated by removing cells from cadaver goat-lung tissue, and the scaffold was modified with chitosan/nanohydroxyapatite composite for the purpose of bone tissue engineering applications. MTT assay with osteoblasts, seeded over the chitosan/ nanohydroxyapatite-modified decellularized scaffold, demonstrated significantly higher cell growth as compared to the decellularized scaffold without modification. SEM analysis of cell-seeded scaffold, after incubation for 7 days, represented a good cell adhesion, and the cells spread over the chitosan/nanohydroxyapatite-modified decellularized scaffold. Expression of bone-tissue-specific osteocalcin gene in the osteoblast cells grown over the chitosan/nanohydroxyapatite-modified decellularized scaffold clearly signifies that the cells maintained their osteoblastic phenotype with the chitosan/nanohydroxyapatite-modified decellularized scaffold. Therefore, it can be concluded that the decellularized goat-lung scaffold-modified with chitosan/nanohydroxyapatite composite, may provide enhanced osteogenic potential when used as a scaffold for bone tissue engineering. © 2013 Sweta K. Gupta et al.
Citation: BioMed Research International (2013), 2013(): -
URI: https://doi.org/10.1155/2013/651945
http://repository.iitr.ac.in/handle/123456789/14789
Issue Date: 2013
ISSN: 23146133
Author Scopus IDs: 56968266700
7005837526
6602889612
36798999600
Author Affiliations: Gupta, S.K., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, UP 247001, India
Dinda, A.K., Department of Pathology, All India Institute of Medical Sciences, New Delhi 110029, India
Potdar, P.D., Department of Molecular Medicine and Biology, Jaslok Hospital and Research Center, Mumbai, Maharashtra 400026, India
Mishra, N.C., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, UP 247001, India
Corresponding Author: Mishra, N.C.; Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, UP 247001, India; email: mishrawise@gmail.com
Appears in Collections:Journal Publications [PE]

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