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Title: Fabrication and characterization of PCL/gelatin composite nanofibrous scaffold for tissue engineering applications by electrospinning method
Authors: Gautam S.
Dinda A.K.
Mishra, Narayan Chandra
Published in: Materials Science and Engineering C
Abstract: In the present study, composite nanofibrous tissue engineering-scaffold consisting of polycaprolactone and gelatin, was fabricated by electrospinning method, using a new cost-effective solvent mixture: chloroform/methanol for polycaprolactone (PCL) and acetic acid for gelatin. The morphology of the nanofibrous scaffold was investigated by using field emission scanning electron microscopy (FE-SEM) which clearly indicates that the morphology of nanofibers was influenced by the weight ratio of PCL to gelatin in the solution. Uniform fibers were produced only when the weight ratio of PCL/gelatin is sufficiently high (10:1). The scaffold was further characterized by Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric (TG) analysis, and X-ray diffraction (XRD). FT-IR and TG analysis indicated some interactions between PCL and gelatin molecules within the scaffold, while XRD results demonstrated crystalline nature of PCL/gelatin composite scaffold. Cytotoxicity effect of scaffold on L929 mouse fibroblast cells was evaluated by MTT assay and cell proliferation on the scaffold was confirmed by DNA quantification. Positive results of MTT assay and DNA quantification L929 mouse fibroblast cells indicated that the scaffold made from the combination of natural polymer (gelatin) and synthetic polymer (PCL) may serve as a good candidate for tissue engineering applications. © 2012 Elsevier B.V. All Rights Reserved.
Citation: Materials Science and Engineering C (2013), 33(3): 1228-1235
Issue Date: 2013
Keywords: Composite scaffold
Tissue engineering
ISSN: 9284931
Author Scopus IDs: 57213372885
Author Affiliations: Gautam, S., Department of Polymer and Process Engineering, IIT Roorkee, Saharanpur Campus, Saharanpur, U.P. 247 001, India
Dinda, A.K., Department of Pathology, All India Institute of Medical Science, New Delhi, 110029, India
Mishra, N.C., Department of Polymer and Process Engineering, IIT Roorkee, Saharanpur Campus, Saharanpur, U.P. 247 001, India
Funding Details: The authors are grateful to Professor Chia-Fu Chou, Institute of Physics, Academia Sinica, Taiwan for his valuable suggestions and encouragement during this work, and Dr. R. Jayaganthan, Associate professor, Department of Metallurgical and Materials Engineering, Indian Institute of Technology Roorkee, for his constant encouragement and suggestions for this work. The financial assistance for this work by Ministry of Human Resource Development (MHRD), Govt. of India, is gratefully acknowledged. We are also thankful to Mr. S.K. Srivastava, Research Scholar, Department of Pathology, AIIMS, New Delhi for his co-operation in carrying out the biocompatibility experiments.
Corresponding Author: Mishra, N.C.; Department of Polymer and Process Engineering, IIT Roorkee, Saharanpur Campus, Saharanpur, U.P. 247 001, India; email:
Appears in Collections:Journal Publications [PE]

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