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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/14728
Title: Fabrication and characterization of novel nano-biocomposite scaffold of chitosan-gelatin-alginate-hydroxyapatite for bone tissue engineering
Authors: Sharma C.
Dinda A.K.
Potdar P.D.
Chou C.-F.
Mishra, Narayan Chandra
Published in: Materials Science and Engineering C
Abstract: A novel nano-biocomposite scaffold was fabricated in bead form by applying simple foaming method, using a combination of natural polymers-chitosan, gelatin, alginate and a bioceramic-nano-hydroxyapatite (nHAp). This approach of combining nHAp with natural polymers to fabricate the composite scaffold, can provide good mechanical strength and biological property mimicking natural bone. Environmental scanning electron microscopy (ESEM) images of the nano-biocomposite scaffold revealed the presence of interconnected pores, mostly spread over the whole surface of the scaffold. The nHAp particulates have covered the surface of the composite matrix and made the surface of the scaffold rougher. The scaffold has a porosity of 82% with a mean pore size of 112 ± 19.0 μm. Swelling and degradation studies of the scaffold showed that the scaffold possesses excellent properties of hydrophilicity and biodegradability. Short term mechanical testing of the scaffold does not reveal any rupturing after agitation under physiological conditions, which is an indicative of good mechanical stability of the scaffold. In vitro cell culture studies by seeding osteoblast cells over the composite scaffold showed good cell viability, proliferation rate, adhesion and maintenance of osteoblastic phenotype as indicated by MTT assay, ESEM of cell-scaffold construct, histological staining and gene expression studies, respectively. Thus, it could be stated that the nano-biocomposite scaffold of chitosan-gelatin-alginate-nHAp has the paramount importance for applications in bone tissue-engineering in future regenerative therapies. © 2016 Elsevier B.V. All rights reserved.
Citation: Materials Science and Engineering C (2016), 64(): 416-427
URI: https://doi.org/10.1016/j.msec.2016.03.060
http://repository.iitr.ac.in/handle/123456789/14728
Issue Date: 2016
Publisher: Elsevier Ltd
Keywords: Biocomposite scaffold
Bone tissue engineering
Foaming
Nano-hydroxyapatite
Natural polymers
Osteoblast
ISSN: 9284931
Author Scopus IDs: 55418158400
7005837526
6602889612
7403593283
36798999600
Author Affiliations: Sharma, C., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, India
Dinda, A.K., Department of Molecular Medicine and Biology, Jaslok Hospital and Research Centre, Mumbai, 400 026, India
Potdar, P.D., Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India
Chou, C.-F., Institute of Physics, Academia Sinica, Taipei, 11529, Taiwan
Mishra, N.C., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, India
Funding Details: The authors are grateful to the Council of Scientific and Industrial Research (Grant No. 27(0222)/10/EM R-II dated 31.05.10), as well as Ministry of Human Resources and Development (MHRD), India, for funding this research work. We are thankful to the Management of Jaslok Hospital and Research Centre, Mumbai for providing facilities to do tissue culture work and Mr. Sachin Ramdas Chaugule, Research Assistant, Dept. of Molecular Medicine and Biology, for his co-operation in carrying out the biocompatibility experiments.
Corresponding Author: Mishra, N.C.; Department of Polymer and Process Engineering, Indian Institute of Technology RoorkeeIndia; email: mishrawise@gmail.com
Appears in Collections:Journal Publications [PE]

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