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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1472
Title: Crystal structure of pentapeptide-independent chemotaxis receptor methyltransferase (CheR) reveals idiosyncratic structural determinants for receptor recognition
Authors: Batra M.
Sharma R.
Malik A.
Dhindwal S.
Kumar P.
Tomar S.
Published in: Journal of Structural Biology
Abstract: Chemotactic methyltransferase, CheR catalyse methylation of specific glutamate residues in the cytoplasmic domain of methyl-accepting chemotactic protein receptors (MCPRs). The methylation of MCPRs is essential for the chemical sensing and chemotactic bacterial mobility towards favorable chemicals or away from unfavorable ones. In this study, crystal structure of B. subtilis CheR (BsCheR) in complex with S-adenosyl-L-homocysteine (SAH) has been determined to 1.8 Å resolution. This is the first report of crystal structure belonging to the pentapeptide-independent CheR (PICheR) class. Till date, only one crystal structure of CheR from S. typhimurium (StCheR) belonging to pentapeptide-dependent CheR (PDCheR) class is available. Structural analysis of BsCheR reveals a helix-X-helix motif (HXH) with Asp53 as the linker residue in the N-terminal domain. The key structural features of the PDCheR ?-subdomain involved in the formation of a tight complex with the pentapeptide binding motif in MCPRs were found to be absent in the structure of BsCheR. Additionally, isothermal titration calorimetry (ITC) experiments were performed to investigate S-adenosyl-(L)-methionine (SAM) binding affinity and KD was determined to be 0.32 mM. The structure of BsCheR reveals that mostly residues of the large C-terminal domain contribute to SAH binding, with contributions of few residues from the linker region and the N-terminal domain. Structural investigations and sequence analysis carried out in this study provide critical insights into the distinct receptor recognition mechanism of the PDCheR and PICheR methyltransferase classes. © 2016 Elsevier Inc.
Citation: Journal of Structural Biology(2016), 196(3): 364-374
URI: https://doi.org/10.1016/j.jsb.2016.08.005
http://repository.iitr.ac.in/handle/123456789/1472
Issue Date: 2016
Publisher: Academic Press Inc.
Keywords: Chemotaxis
Isothermal titration calorimetry
Methylation
Methyltransferase
ISSN: 10478477
Author Scopus IDs: 57188933381
57209347974
57191902133
36082537700
55064809000
57203506001
Author Affiliations: Batra, M., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, India
Sharma, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, India
Malik, A., Department of Biotechnology, Indi
Funding Details: This study was financially supported by the Uttarakhand Council for Biotechnology , Department of Biotechnology, Government of Uttarakhand (Project Ref No. UCB/HLD/15/273 to ST). UGC is acknowledged by MB for her senior research fellowship (SRF), DBT is a
Corresponding Author: Tomar, S.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: shailfbt@iitr.ac.in
Appears in Collections:Journal Publications [BT]

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