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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/14684
Title: Development of a nanocomposite scaffold of gelatin–alginate–graphene oxide for bone tissue engineering
Authors: Purohit S.D.
Bhaskar R.
Singh H.
Yadav I.
Gupta M.K.
Mishra, Narayan Chandra
Published in: International Journal of Biological Macromolecules
Abstract: Bone damage, due to congenital defects, trauma and sports-related injuries, has become a foremost health problem all over the world. The present study involves fabrication of a nanocomposite scaffold of graphene oxide (GO), gelatin and alginate, with an aim of enhancing bone regeneration. The effect of varying concentration of GO on the scaffold properties was also determined. The incorporation of GO enhanced the compressive strength of the nanocomposite scaffolds significantly compared to the gelatin-alginate (GA) scaffold which is without GO. High % swelling (~700%) of the nanocomposite scaffold indicates its high hydrophilicity, which is suitable for tissue engineering. Slow biodegradation (~30% in 28 days) indicates its suitability for bone regeneration. In vitro studies, by seeding MG-63 cells over the nanocomposite scaffolds, revealed an enhancement in cell attachment and proliferation as compared to the GA scaffold: this indicates the positive effect of the GO on the scaffold properties which, in turn, can enhance bone regeneration. Cell differentiation studies, with the mesenchymal stem cells seeded scaffolds, revealed higher expression of osteoblast transcription factors (Runx2 and Osteocalcin) and alkaline phosphatase activity―indicating the scaffold to be a good osteoinductive material. Thus, the nanocomposite scaffold will be a potential scaffold for bone tissue engineering. © 2019
Citation: International Journal of Biological Macromolecules (2019), 133(): 592-602
URI: https://doi.org/10.1016/j.ijbiomac.2019.04.113
http://repository.iitr.ac.in/handle/123456789/14684
Issue Date: 2019
Publisher: Elsevier B.V.
Keywords: Bone tissue engineering
Graphene oxide
Nanocomposite
ISSN: 1418130
Author Scopus IDs: 57209288373
57209288897
57193340084
57188624480
15127016500
36798999600
Author Affiliations: Purohit, S.D., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, India
Bhaskar, R., Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Rourkela, India
Singh, H., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, India
Yadav, I., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, India
Gupta, M.K., Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Rourkela, India
Mishra, N.C., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Roorkee, India
Funding Details: The authors are grateful to the Ministry of Human Resources and Development (MHRD), India, for funding this research work.
Corresponding Author: Mishra, N.C.; Department of Polymer and Process Engineering, Indian Institute of Technology RoorkeeIndia; email: misrafpt@iitr.ac.in
Appears in Collections:Journal Publications [PE]

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