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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1468
Title: Transfer of useful variability of high grain iron and zinc from Aegilops kotschyi into wheat through seed irradiation approach
Authors: Verma S.K.
Kumar S.
Sheikh I.
Malik S.
Mathpal P.
Chugh V.
Kumar S.
Prasad R.
Dhaliwal H.S.
Published in: International Journal of Radiation Biology
Abstract: Purpose To transfer the 2S chromosomal fragment(s) of Aegilops kotschyi (2Sk) into the bread wheat genome which could lead to the biofortification of wheat with high grain iron and zinc content.Materials and methods Wheat-Ae. kotschyi 2A/2Sk substitution lines with high grain iron and zinc content were used to transfer the gene/loci for high grain Fe and Zn content into wheat using seed irradiation approach.Results Bread wheat plants derived from 40 krad-irradiated seeds showed the presence of univalents and multivalents during meiotic metaphase-I. Genomic in situ hybridization analysis of seed irradiation hybrid F2 seedlings showed several terminal and interstitial signals indicated the introgression of Ae. kotschyi chromosome segments. This proves the efficacy of seed radiation hybrid approach in gene transfer experiments. All the radiation-treated hybrid plants with high grain Fe and Zn content were analyzed with wheat group 2 chromosome-specific polymorphic simple sequence repeat markers to identify the introgression of small alien chromosome fragment(s).Conclusion Radiation-induced hybrids showed more than 65% increase in grain iron and 54% increase in Zn contents with better harvest index than the elite wheat cultivar WL711 indicating effective and compensating translocations of 2Sk fragments into wheat genome. © 2016 Taylor and Francis.
Citation: International Journal of Radiation Biology (2016), 92(3): 132-139
URI: https://doi.org/10.3109/09553002.2016.1135263
http://repository.iitr.ac.in/handle/123456789/1468
Issue Date: 2016
Publisher: Taylor and Francis Ltd
Keywords: Biofortification
iron
seed irradiation
wheat
zinc
ISSN: 9553002
Author Scopus IDs: 55303446800
55500537400
56596417500
56596051700
56596836300
44860952300
8982913900
16690394400
35466824500
Author Affiliations: Verma, S.K., School of Life Sciences, Central University of Himachal Pradesh, Dharamshala, Kangra, HP, 176 206, India, Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Kumar, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Sheikh, I., Akal College of Agriculture, Eternal University, Baru-Sahib, Sirmour, HP, India
Malik, S., Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
Mathpal, P., Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
Chugh, V., Akal College of Agriculture, Eternal University, Baru-Sahib, Sirmour, HP, India
Kumar, S., Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
Prasad, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Dhaliwal, H.S., Akal College of Agriculture, Eternal University, Baru-Sahib, Sirmour, HP, India
Corresponding Author: Verma, S.K.; School of Life Sciences, Central University of Himachal PradeshIndia; email: skverma@cuhimachal.ac.in
Appears in Collections:Journal Publications [BT]

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