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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1466
Title: Modulation of Candida albicans Biofilm by Different Carbon Sources
Authors: Pemmaraju S.C.
Pruthi P.A.
Prasad, Ramasare A.
Pruthi V.
Published in: Mycopathologia
Abstract: In the present investigation, the role of carbon sources (glucose, lactate, sucrose, and arabinose) on Candida albicans biofilm development and virulence factors was studied on polystyrene microtiter plates. Besides this, structural changes in cell wall component ?-glucan in presence of different carbon sources have also been highlighted. Biofilm formation was analyzed by XTT (2,3-bis[2-Methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) reduction assay. Glucose-grown cells exhibited the highest metabolic activity during adhesion among all carbon sources tested (p < 0.05). However, cells exposed to sucrose exhibited highest biofilm formation and matrix polysaccharides secretion after 48 h. The results also correlated with the biofilm height and roughness measurements by atomic force microscopy. Exposure to lactate induced hyphal structures with the highest proteinase activity while arabinose-grown cells formed pseudohyphal structures possessing the highest phospholipase activity. Structural changes in ?-glucan characterized by Fourier transform infrared (FTIR) spectroscopy displayed characteristic band of ?-glucan at 892 cm?1in all carbon sources tested. The ?(1?6) to ?(1?3) glucan ratio calculated as per the band area of the peak was less in lactate (1.15) as compared to glucose (1.73), sucrose (1.62), and arabinose (2.85). These results signify that carbon sources influence C. albicans biofilm development and modulate virulence factors and structural organization of cell wall component ?-glucan. © 2016, Springer Science+Business Media Dordrecht.
Citation: Mycopathologia (2016), 181(43987): 341-352
URI: https://doi.org/10.1007/s11046-016-9992-8
http://repository.iitr.ac.in/handle/123456789/1466
Issue Date: 2016
Publisher: Springer Netherlands
Keywords: Biofilm
Candida albicans
FTIR
Virulence
XTT assay
?-glucan
ISSN: 0301486X
Author Scopus IDs: 54684894200
25958285300
16690394400
18837702800
Author Affiliations: Pemmaraju, S.C., Molecular Microbiology Lab, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT Roorkee), Roorkee, Uttarakhand 247667, India
Pruthi, P.A., Molecular Microbiology Lab, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT Roorkee), Roorkee, Uttarakhand 247667, India
Prasad, R., Molecular Biology and Proteomics Lab, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT Roorkee), Roorkee, Uttarakhand 247667, India
Pruthi, V., Molecular Microbiology Lab, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT Roorkee), Roorkee, Uttarakhand 247667, India
Corresponding Author: Pruthi, V.; Molecular Microbiology Lab, Department of Biotechnology, Indian Institute of Technology Roorkee (IIT Roorkee)India; email: vikasfbs@gmail.com
Appears in Collections:Journal Publications [BT]

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