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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1465
Title: Impact of oxidative and osmotic stresses on Candida albicans biofilm formation
Authors: Pemmaraju S.C.
Padmapriya K.
Pruthi P.A.
Prasad, Ramasare A.
Pruthi V.
Published in: Biofouling
Abstract: Candida albicans possesses an ability to grow under different host-driven stress conditions by developing robust protective mechanisms. In this investigation the focus was on the impact of osmotic (2M NaCl) and oxidative (5 mM H2O2) stress conditions during C. albicans biofilm formation. Oxidative stress enhanced extracellular DNA secretion into the biofilm matrix, increased the chitin level, and reduced virulence factors, namely phospholipase and proteinase activity, while osmotic stress mainly increased extracellular proteinase and decreased phospholipase activity. Fourier transform infrared and nuclear magnetic resonance spectroscopy analysis of mannan isolated from the C. albicans biofilm cell wall revealed a decrease in mannan content and reduced ?-linked mannose moieties under stress conditions. The results demonstrate that C. albicans adapts to oxidative and osmotic stress conditions by inducing biofilm formation with a rich exopolymeric matrix, modulating virulence factors as well as the cell wall composition for its survival in different host niches. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
Citation: Biofouling (2016), 32(8): 897-909
URI: https://doi.org/10.1080/08927014.2016.1212021
http://repository.iitr.ac.in/handle/123456789/1465
Issue Date: 2016
Publisher: Taylor and Francis Ltd.
Keywords: biofilm
Candida albicans
eDNA
osmotic stress
oxidative stress
ISSN: 8927014
Author Scopus IDs: 54684894200
57190815877
25958285300
16690394400
18837702800
Author Affiliations: Pemmaraju, S.C., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Padmapriya, K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Pruthi, P.A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Prasad, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Pruthi, V., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India
Corresponding Author: Pruthi, V.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: vikasfbs@iitr.ac.in
Appears in Collections:Journal Publications [BT]

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