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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/14630
Title: Amaranth seeds (Amaranthus palmeri L.) as novel feedstock for biodiesel production by oleaginous yeast
Authors: Deeba F.
Patel A.
Arora N.
Pruthi V.
Pruthi P.A.
Negi, Yuvraj Singh.
Published in: Environmental Science and Pollution Research
Abstract: The potential of lipid accumulation by oleaginous yeast Cryptococcus vishniaccii grown on amaranth seed aqueous extract (AAE) media was assessed. Maximum cell biomass productivity of 104 mg/L/h, lipid productivity of 54 mg/L/h, and lipid content of 52.31% were recorded on AAE when carbon to nitrogen (C:N) ratio increased from 134 to 147 after removal of ammonia nitrogen. The lipid droplet (LD) size (2.32 ± 0.38 μm) was visualized by fluorescence microscopy using Nile red stain indicating maximum accumulated triacylglycerol (TAG) at C:N 147. Fatty acid methyl ester (FAME) profile obtained after transesterification of extracted lipid revealed the presence of palmitic acid (16:0), palmitoleic acid (16:1), stearic acid (18:0), oleic acid (18:1), and linoleic acid (18:2). Data showed the presence of high monounsaturated fatty acid (MUFA) content (68.17%) depicting improved winter operating conditions of biodiesel. Various quality parameters of biodiesel were evaluated and compared to the American and European biodiesel standards specifications. Based on the lipid productivity, distribution of fatty acids, and evaluated properties obtained; the lipid accumulation by C. vishniaccii utilizing amaranth seeds as substrate could serve as a feasible feedstock for biodiesel production. © 2017, Springer-Verlag GmbH Germany.
Citation: Environmental Science and Pollution Research (2018), 25(1): 353-362
URI: https://doi.org/10.1007/s11356-017-0444-x
http://repository.iitr.ac.in/handle/123456789/14630
Issue Date: 2018
Publisher: Springer Verlag
Keywords: Amaranth seed aqueous extract (AAE)
Biodiesel
Carbon-to-nitrogen (C:N) ratio
Cryptococcus vishniaccii
Lipid productivity
Nile red
ISSN: 9441344
Author Scopus IDs: 56113434700
55930061600
56800005300
18837702800
25958285300
6701821524
Author Affiliations: Deeba, F., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, Uttar Pradesh 247001, India
Patel, A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Arora, N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Pruthi, V., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Pruthi, P.A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Negi, Y.S., Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, Uttar Pradesh 247001, India
Funding Details: Acknowledgements This research work was financially supported by the Ministry of Human Resource Development (MHRD), Government of India.
Corresponding Author: Negi, Y.S.; Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, India; email: yuvrajnegi@gmail.com
Appears in Collections:Journal Publications [PE]

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