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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1461
Title: Aspergillus Niger PA2: A novel strain for extracellular biotransformation of l-tyrosine into l-DOPA
Authors: Agarwal P.
Pareek N.
Dubey S.
Singh J.
Singh, Rajesh Pratap
Published in: Amino Acids
Abstract: l-DOPA (3,4-dihydroxyphenyl-l-alanine), an amino acid derivative is the most widely used drug of choice for the treatment of Parkinson's disease and other neurologic injuries. The present study deals with the elevated biochemical transformation of l-tyrosine to l-DOPA by Aspergillus niger PA2, a potent tyrosinase producer, isolated from decomposed food wastes. This appears to be the first report on A. niger as a notable extracellular tyrosinase producer. The extracellular tyrosinase activity produced remarkably higher levels of l-DOPA, i.e. 2.44 mg mL-1 when the media was supplemented with 5 mg mL-1 l-tyrosine. The optimum pH for tyrosinase production was 6.0, with the maximal l-DOPA production at the same pH. The product thus produced was analyzed by thin-layer chromatography, UV spectroscopy, high-performance liquid chromatography and Fourier transform infrared spectroscopy, that had denoted this to be l-DOPA. Kinetic parameters viz. Y p/s, Q s and Q p had further indicated the notable levels of production. Thus, Aspergillus niger PA2 could be a promising resource and may be further exploited for large-scale production of l-DOPA. © 2016 Springer-Verlag Wien.
Citation: Amino Acids(2016), 48(5): 1253-1262
URI: https://doi.org/10.1016/j.bpc.2016.09.006
http://repository.iitr.ac.in/handle/123456789/1461
Issue Date: 2016
Publisher: Springer-Verlag Wien
Keywords: Aspergillus Niger
Biotransformation
l-DOPA
Tyrosinase
ISSN: 9394451
Author Scopus IDs: 55668760800
35741588300
57208768197
57213875577
57208931324
Author Affiliations: Agarwal, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, India
Pareek, N., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, India, Department of Microbiology, Central Univers
Funding Details: Research fellowships awarded to PA by University Grant Commission, Govt. of India, to NP by Council of Scientific and Industrial Research, Govt. of India, to SS by Department of Biotechnology Govt. of India and to JS by Ministry of Human Resource Developm
Corresponding Author: Singh, R.P.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: rpsbsfbs@iitr.ac.in
Appears in Collections:Journal Publications [BT]

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