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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1448
Title: Induced Homoeologous Pairing for Transfer of Useful Variability for High Grain Fe and Zn from Aegilops kotschyi into Wheat
Authors: Verma S.K.
Kumar S.
Sheikh I.
Sharma P.
Mathpal P.
Malik S.
Kundu P.
Awasthi A.
Kumar S.
Prasad R.
Dhaliwal H.S.
Published in: Plant Molecular Biology Reporter
Abstract: Biofortification of bread wheat by the transfer of useful variability of high grain Fe and Zn from Aegilops kotschyi through induced homoeologous pairing is the most feasible approach to alleviate micronutrient malnutrition worldwide. Deficiency of chromosome 5B in interspecific hybrids allows homoeologous pairing and recombination of chromosomes of wheat with those of the related species. The interspecific hybrid plants without 5B chromosome showed much higher chromosome pairing than did the plants with 5B. The F1 plants without 5B chromosome were selected and repeatedly backcrossed with wheat cultivar PBW343. The chromosome number of BC2F1 plants ranged from 43 to 60 with several univalents and multivalents. Molecular markers and GISH analysis confirmed the introgression of U/S chromosomes of Ae. kotschyi and their fragments in wheat. The BC2F2 plants showed up to 125 % increase in Fe and 158 % increase in Zn compared to PBW343 with Lr24 and Yr36. Induced homoeologous pairing in the absence of 5B was found to be an effective approach for transfer of useful variability for enhanced grain Fe and Zn content for biofortification of wheat for high grain micronutrient content. © 2016, Springer Science+Business Media New York.
Citation: Plant Molecular Biology Reporter (2016), 34(6): 1083-1094
URI: https://doi.org/10.1007/s11105-016-0989-8
http://repository.iitr.ac.in/handle/123456789/1448
Issue Date: 2016
Publisher: Springer New York LLC
Keywords: Aegilops
Chromosome 5B
Homoeologous recombination
Iron
Wheat
Zinc
ISSN: 7359640
Author Scopus IDs: 55303446800
55500537400
56596417500
56317123400
56596836300
56596051700
57188985466
37014402100
8982913900
16690394400
35466824500
Author Affiliations: Verma, S.K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247 667, India, School of Life Sciences, Central University of Himachal Pradesh, Dharamshala, Himachal Pradesh 176 215, India
Kumar, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247 667, India
Sheikh, I., Akal College of Agriculture, Eternal University, Baru-Sahib, Sirmour, Himachal Pradesh 173 101, India
Sharma, P., Akal College of Agriculture, Eternal University, Baru-Sahib, Sirmour, Himachal Pradesh 173 101, India
Mathpal, P., Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G B Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145, India
Malik, S., Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G B Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145, India
Kundu, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247 667, India
Awasthi, A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247 667, India
Kumar, S., Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G B Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145, India
Prasad, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247 667, India
Dhaliwal, H.S., Akal College of Agriculture, Eternal University, Baru-Sahib, Sirmour, Himachal Pradesh 173 101, India
Funding Details: Authors are highly thankful to the Department of Biotechnology, Govt. of India for supporting the work through project "Biofortification of Wheat for enhanced Iron and Zinc content by conventional and molecular breeding???Phase-II" (BT/AGR/Wheat Bioforti/PH-II/2010). SKV and SK are highly grateful to the Council for Scientific and Industrial Research for providing research fellowships.
Corresponding Author: Verma, S.K.; School of Life Sciences, Central University of Himachal PradeshIndia; email: skverma.cuhl@nic.in
Appears in Collections:Journal Publications [BT]

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