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Title: Augmented lipid accumulation in ethyl methyl sulphonate mutants of oleaginous microalga for biodiesel production
Authors: Mehtani J.
Arora N.
Patel A.
Jain P.
Pruthi P.A.
Poluri, Krishna Mohan
Pruthi V.
Published in: Bioresource Technology
Abstract: The aim of this work was to generate high lipid accumulating mutants of Chlorella minutissima (CM) using ethyl methyl sulphonate (EMS) as a random chemical mutagen. Amid the 5% surviving cells after exposure to EMS (2 M), three fast growing mutants (CM2, CM5, CM7) were selected and compared with wild type for lipid productivity and biochemical composition. Among these mutants, CM7 showed the maximum biomass (2.4 g/L) and lipid content (42%) as compared to wild type (1.5 g/L; 27%). Further, the mutant showed high photosynthetic pigments with low starch content signifying the re-allocation of carbon flux to lipid. The obtained mutant showed no visible morphological changes in comparison to its WT. The fatty acid profile showed increase in monounsaturated fatty acids while decreased saturated and polyunsaturated fatty acids signifying good quality biodiesel. The mutant strain thus obtained can be optimized further and applied for enhanced biodiesel production. © 2017 Elsevier Ltd
Citation: Bioresource Technology(2017), 242(): 121-127
Issue Date: 2017
Publisher: Elsevier Ltd
Keywords: Chlorella minutissima
Ethyl Methyl Sulphonate
Lipid content
ISSN: 9608524
Author Scopus IDs: 57193769468
Author Affiliations: Mehtani, J., Department of Biotechnology, Indian Institute of Technology Roorkee, India
Arora, N., Department of Biotechnology, Indian Institute of Technology Roorkee, India
Patel, A., Department of Biotechnology, Indian Institute of Technology Roorke
Funding Details: Authors are thankful for financial support by the Department of Biotechnology, Govt. of India, BioCare Programme, DBT Sanction No. 102/IFD/SAN/3539/2011-2012 (Grant No. DBT-608-BIO) to PAP and DBT-SRF to NA (Grant No. 7001-35-44). KMP acknowledges the rec
Corresponding Author: Pruthi, V.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email:
Appears in Collections:Journal Publications [BT]

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