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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1414
Title: Putative protein VC0395_0300 from Vibrio cholerae is a diguanylate cyclase with a role in biofilm formation
Authors: Bandekar D.
Chouhan O.P.
Mohapatra S.
Hazra M.
Hazra S.
Biswas S.
Published in: Microbiological Research
Abstract: The hallmark of the lifecycle of Vibrio cholerae is its ability to switch between two lifestyles ? the sessile, non-pathogenic form and the motile, infectious form in human hosts. One of these changes is in the formation of surface biofilms, when in sessile aquatic habitats. The cell–cell interactions within a V. cholerae biofilm are stabilized by the production of an exopolysachharide (EPS) matrix, which in turn is regulated by the ubiquitous secondary messenger, cyclic di-GMP (c-di-GMP), synthesized by proteins containing GGD(/E)EF domains in all prokaryotic systems. Here, we report the functional role of the VC0395_0300 protein (Sebox3) encoded by the chromosome I of V. cholerae, with a GGEEF signature sequence, in the formation of surface biofilms. In our study, we have shown that Escherichia coli containing the full-length Sebox3 displays enhanced biofilm forming ability with cellulose production as quantified and visualized by multiple assays, most notably using FEG-SEM. This has also been corroborated with the lack of motility of host containing Sebox3 in semi-solid media. Searching for the reasons for this biofilm formation, we have demonstrated in vitro that Sebox3 can synthesize c-di-GMP from GTP. The homology derived model of Sebox3 displayed significant conservation of the GGD(/E)EF architecture as well. Hence, we propose that the putative protein VC0395_0300 from V. cholerae is a diguanylate cyclase which has an active role in biofilm formation. © 2017 Elsevier GmbH
Citation: Microbiological Research (2017), 202(): 61-70
URI: https://doi.org/10.1016/j.micres.2017.05.003
http://repository.iitr.ac.in/handle/123456789/1414
Issue Date: 2017
Publisher: Elsevier GmbH
Keywords: Biofilm
c-di-GMP
Diguanylate cyclase
GGEEF domain
Motility
ISSN: 9445013
Author Scopus IDs: 57038939400
57039239200
56849949200
57039252100
15837197000
24786148800
Author Affiliations: Bandekar, D., VISTA Lab, BITS, Pilani ? K K Birla Goa Campus, Zuarinagar, Goa, India
Chouhan, O.P., VISTA Lab, BITS, Pilani ? K K Birla Goa Campus, Zuarinagar, Goa, India
Mohapatra, S., Department of Biotechnology, Indian Institute of Technology RoorkeeUttarakhand, India
Hazra, M., Department of Botany and Microbiology, Gurukula Kangri University, Haridwar, Uttarakhand, India
Hazra, S., Department of Biotechnology, Indian Institute of Technology RoorkeeUttarakhand, India, Centre for Nanotechnology, Indian Institute of Technology RoorkeeUttarakhand, India
Biswas, S., VISTA Lab, BITS, Pilani ? K K Birla Goa Campus, Zuarinagar, Goa, India
Funding Details: The authors wish to acknowledge BRNS, DAE for a research grant and for providing a fellowship to DB. We also express sincere thanks to SERB, DST for providing a research grant which aided in part funding of this study. The authors also wish to thank Institute Instrumentation Centre (IIC) and ICC, IIT-Roorkee and Bioinformatics Resources and Applications Facility (BRAF), C-DAC, Pune for the provision of experimental and computational facilities and support.
Corresponding Author: Biswas, S.; VISTA Lab, BITS, Pilani ? K K Birla Goa CampusIndia; email: sumit@goa.bits-pilani.ac.in
Appears in Collections:Journal Publications [BT]

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