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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1410
Title: In Vitro Apoptosis Induction in a Human Prostate Cancer Cell Line by Thermotolerant Glycolipid from Bacillus licheniformis SV1
Authors: Gupta S.
Varshney R.
Jha R.K.
Pruthi P.A.
Roy, Partha
Pruthi V.
Published in: Journal of Surfactants and Detergents
Abstract: A highly potent glycolipid was isolated from a crude oil-contaminated soil bacterium Bacillus licheniformis SV1 (NCBI GenBank Acc. No. KX130852) when grown on a modified mineral salt medium supplemented with 2% oleic acid. The maximum reduction in surface tension of cell-free broth from 71 ± 0.812 to 25.919 ± 0.984 mN/m with 89 ± 0.346% emulsification activity was recorded after 120 h of growth. Glycolipid was purified using chromatographic techniques and the presence of aliphatic chain (C–H stretch) and OH-band was revealed by NMR, GC–MS and FTIR analysis. Stability of glycolipid up to 250 °C and its complete decomposition at 507 °C was recorded by thermogravimetric analysis (TGA). MTT assay (IC50 = 0.473 ± 0.048 mg/ml) along with 4?,6-diamidino-2-phenylindole (DAPI) and acridine orange and ethidium bromide (AO/EtBr) staining validated that glycolipid induces cell apoptosis in human prostate cancer cell line (PC-3). Furthermore, potent anti-cancerous compounds such as 9-octadecanoic acid (22.55%), linoleic acid methyl ester (2.2%) and palmitic acid (1.18%) were also detected in the GC–MS spectra of the purified glycolipid. © 2017, AOCS.
Citation: Journal of Surfactants and Detergents (2017), 20(5): 1141-1151
URI: https://doi.org/10.1007/s11743-017-1986-0
http://repository.iitr.ac.in/handle/123456789/1410
Issue Date: 2017
Publisher: Springer Verlag
Keywords: Bacillus licheniformis
Glycolipid
Prostate cancer
Thermogravimetric analysis
ISSN: 10973958
Author Scopus IDs: 57209786212
57191823442
57205264725
25958285300
35509207200
18837702800
Author Affiliations: Gupta, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Varshney, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Jha, R.K., Evolutionary and Medical Genetics Lab, CCMB, Hyderabad, India
Pruthi, P.A., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Roy, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Pruthi, V., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Funding Details: Authors are highly grateful to the Department of Science and Technology, INSPIRE (DST, INSPIRE) Govt. of India (Grant No. IF130678) for providing financial assistance to carry out the present work. Authors are also thankful to Institute Instrumentation Centre (IIC), IIT Roorkee and Department of Chemical Engineering, IIT Roorkee for providing the instrumentation facility.
Corresponding Author: Pruthi, V.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: vikasfbs@gmail.com
Appears in Collections:Journal Publications [BT]

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