Skip navigation
Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1386
Title: Effect of intermittent aeration on microbial diversity in an intermittently aerated IFAS reactor treating municipal wastewater: A field study
Authors: Bhatia A.
Singh N.K.
Bhando T.
Pathania, Ranjana
Kazmi, Absar Ahmad
Published in: Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Abstract: In the present study, the effect of three intermittent aeration (IA) cycles on treatment performance and microbial diversity was investigated in an integrated fixed film activated sludge (IFAS) reactor treating municipal wastewater. The results showed that IA strategies were able to achieve efficient removal of organics and nitrogen ranging between 90 and 95% and 70 and 80%, respectively, however the phosphorus removal was found to be inversely proportional to the duration of aeration off time in each IA cycle. The microscopic analysis revealed that the suspended and attached biomass had compact morphology and open floc structure, respectively. For each gram of volatile suspended solids, 165 and 148 mg of extracellular polymeric substances (EPS) were extracted from attached and suspended biomass, respectively, constituting carbohydrates (?24%), proteins (?31%), humic acids (?28%), DNA (?2%) and unknown substances (?12%). The microbial diversities of suspended biomass in IFAS reactor were investigated using culture-dependent approach, which confirmed the presence of Clostridium spp., Pseudomonas spp., Bacillus spp., Escherichia coli spp., Nitrosococcus spp., Streptococcus spp., Acinetobacter spp., Betaproteobacteria outliers, Klebsiella pneumoniae, Klebsiella aerogenes, Serratia marcescens, Micrococcus, Proteus vulgaris spp., Actinomycetes spp., and Actinobacteria including Micromonospora spp. and Streptomyces spp. Molecular tools for diversity analyses were used for ammonia and nitrite oxidizer identification, such as Nitrospira and Nitrosococcus species. Denitrifiers include the species of Pseudomonas, Betaproteobacteria and Flavobacterium. Acinetobacter, Betaproteobacteria and Gammaproteobacteria were responsible for the phosphorus removal in the present system. Overall, the system performed efficiently showing Proteobacteria (59%), Acinetobacter (12%) and Bacteroidetes (11%) as the dominant bacterial groups. However, the dominance of the bacterial diversity varied with the IA cycle time numerating the maximum percentage of bacterial species during IA1 phase i.e. 2.5 h aeration/0.5 h non-aeration. © 2017 Taylor & Francis Group, LLC.
Citation: Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering (2017), 52(5): 440-448
URI: https://doi.org/10.1080/10934529.2016.1271665
http://repository.iitr.ac.in/handle/123456789/1386
Issue Date: 2017
Publisher: Taylor and Francis Inc.
Keywords: 16S rRNA
EPS
IFAS
microbial community
nutrient removal
SEM
ISSN: 10934529
Author Scopus IDs: 44760896800
57214658978
56266736600
7004308029
6701514485
Author Affiliations: Bhatia, A., Department of Civil Engineering, Indian Institute of Technology, Roorkee, India
Singh, N.K., Department of Civil Engineering, Indian Institute of Technology, Roorkee, India
Bhando, T., Department of Biotechnology, Indian Institute of Technology, Roorkee, India
Pathania, R., Department of Biotechnology, Indian Institute of Technology, Roorkee, India
Kazmi, A.A., Department of Civil Engineering, Indian Institute of Technology, Roorkee, India
Corresponding Author: Singh, N.K.; Department of Civil Engineering, Indian Institute of TechnologyIndia; email: nitin261187@gmail.com
Appears in Collections:Journal Publications [BT]

Files in This Item:
There are no files associated with this item.
Show full item record


Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.