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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1375
Title: Potential of Trametes maxima IIPLC-32 derived laccase for the detoxification of phenolic inhibitors in lignocellulosic biomass prehydrolysate
Authors: Suman S.K.
Khatri M.
Dhawaria M.
Kurmi A.
Pandey D.
Ghosh, Sanjoy
Jain S.L.
Published in: International Biodeterioration and Biodegradation
Abstract: Lignin derived inhibitors have a detrimental effect on biomass hydrolysis making the use of lignocellulosic biomass irrelevant at industrial scale. Laccase mediated removal of inhibitors has generated significant interest owing to its wide range of substrate specificity, low cost and environment-friendly nature making it an appropriate choice for the biotechnology industry. In the present work, a white rot fungal strain isolated from decaying wood samples was characterized for laccase production using a dual screening approach with ABTS (2,2?-azino-bis(3-ethylbenzothiazoline-6-sulfonate)) and methylene blue as substrates. Extracellular laccase was purified from this strain using anion exchange chromatography and observed to have molecular mass of 70 kDA. The UV–vis spectra of the purified enzyme confirmed blue type laccase. The enzyme exhibited stability in the pH range 2–5 and temperature of 50–70 °C with ABTS. The enzyme also displayed elevated level of tolerance to inhibitors like sodium azide and dithiothreitol. Purified laccase showed Km and Kcat value of 59.73 ?M and 1168 sec?1 respectively with ABTS and 16.29 ?M, 84.3 sec?1 using syringaldazine as substrate respectively. The 0.1U/ml of Trametes maxima IIPLC-32 laccase removed 66% of lignin-derived soluble phenolic inhibitors from sugarcane bagasse within half hour at 5.5 pH. These properties indicate a high potential of Trametes maxima IIPLC-32 laccase for industrial applications. © 2018 Elsevier Ltd
Citation: International Biodeterioration and Biodegradation (2018), 133(): 1-8
URI: https://doi.org/10.1016/j.ibiod.2018.05.009
http://repository.iitr.ac.in/handle/123456789/1375
Issue Date: 2018
Publisher: Elsevier Ltd
Keywords: Inhibitor
Laccase
Lignin
Lignocellulose
Prehydrolysate
ISSN: 9648305
Author Scopus IDs: 57211546956
57193269975
56708742400
56663182400
57206496988
56603000600
7404324623
Author Affiliations: Suman, S.K., CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248005, India, Indian Institute of Technology, Roorkee, India
Khatri, M., CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248005, India
Dhawaria, M., CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248005, India
Kurmi, A., CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248005, India
Pandey, D., CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248005, India
Ghosh, S., Indian Institute of Technology, Roorkee, India
Jain, S.L., CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, 248005, India
Funding Details: Authors are thankful to Director Dr. Anjan Ray, CSIR-Indian Institute of Petroleum for providing necessary facilities to complete this work and constant encouragement. This research was funded by CSIR-IIP as in-house project under OLP-981 .
Corresponding Author: Jain, S.L.; CSIR-Indian Institute of Petroleum, Mohkampur, India
Appears in Collections:Journal Publications [BT]

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