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Title: Molecular cloning and biophysical characterization of CXCL3 chemokine
Authors: Gulati K.
Gangele K.
Agarwal N.
Jamsandekar M.
Kumar D.
Poluri, Krishna Mohan
Published in: International Journal of Biological Macromolecules
Abstract: CXCL3 is a neutrophil activating chemokine that belongs to GRO subfamily of CXC chemokines. GRO chemokine family comprises of three chemokines GRO ? (CXCL1), GRO? (CXCL2), and GRO ? (CXCL3), which arose as a result of gene duplication events during the course of chemokine evolution. Although primary sequences of GRO chemokines are highly similar, they performs several protein specific functions in addition to their common property of neutrophil trafficking. However, the molecular basis for their differential functions has not well understood. Although structural details are available for CXCL1 and CXCL2, no such information regarding CXCL3 is available till date. In the present study, we have successfully cloned, expressed, and purified the recombinant CXCL3. Around 15 mg/L of pure recombinant CXCL3 protein was obtained. Further, we investigated its functional divergence and biophysical characteristics such as oligomerization, thermal stability and heparin binding etc., and compared all these features with its closest paralog CXCL2. Our studies revealed that, although overall structural and oligomerization features of CXCL3 and CXCL2 are similar, prominent differences were observed in their surface characteristics, thus implicating for a functional divergence. © 2017 Elsevier B.V.
Citation: International Journal of Biological Macromolecules(2018), 107(PartA): 575-584
Issue Date: 2018
Publisher: Elsevier B.V.
Keywords: CXCL3
Functional divergence
GRO chemokines
Heparin binding
Molecular evolution
ISSN: 1418130
Author Scopus IDs: 56998446900
Author Affiliations: Gulati, K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Gangele, K., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Agarwal, N., Dep
Funding Details: KMP acknowledge the receipt of DBT-IYBA-BT/07/IYBA/2013-19, and SERB-SB/YS/LS-380/2013 grants from MHz NMR facility at CMBR-Lucknow, and AKTA Prime instrument with Zinc detector from Mr. Sudhir Sharma, GE Healthcare Pvt. Ltd. KMP dedicate this article to
Corresponding Author: Poluri, K.M.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email:
Appears in Collections:Journal Publications [BT]

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