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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/1350
Title: Oxygen uptake rate as a tool for on-line estimation of cell biomass and bed temperature in a novel solid-state fermentation bioreactor
Authors: Arora S.
Singh P.
Rani R.
Ghosh S.
Published in: Bioprocess and Biosystems Engineering
Abstract: Direct measurement of cell biomass is difficult in a solid-state fermentation (SSF) process involving filamentous fungi since the mycelium and the solid substrate are often inseparable. However, respiratory data are rich in information for real-time monitoring of microbial biomass production. In this regard, a correlation was obtained between oxygen uptake rate (OUR) and biomass concentration (X) of Rhizopus oryzae MTCC 1987, during phytase production, in an intermittently mixed novel SSF bioreactor. To obtain the correlation, various models describing sigmoidal growth were tested, namely the logistic, Gompertz, Stannard, and Schnute models. Regression analysis of experimental results, at different operating conditions of inlet air flow rate and relative humidity suggested that OUR and X were correlated well by the logistic model (R2 > 0.90). To corroborate the use of respiratory data for on-line measurement of metabolic activity, OUR was related to metabolic heat generation rate (Rq), and the logistic model was found to satisfactorily correlate Rq and X as well. The model parameter, YQ/X, when substituted into a heat transfer design equation, along with the values of other parameters and operating variables, gave reliable estimates of bed temperature. The correlations developed in the present study, between respiratory activity and biomass concentration may be extended on to other SSF processes for further validation and real-time monitoring of cell biomass and bed temperature. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
Citation: Bioprocess and Biosystems Engineering (2018), 41(7): 917-929
URI: https://doi.org/10.1007/s00449-018-1923-2
http://repository.iitr.ac.in/handle/123456789/1350
Issue Date: 2018
Publisher: Springer Verlag
Keywords: Bed temperature
Cell biomass
OUR
Phytase
Solid-state fermentation
SSF bioreactor
ISSN: 16157591
Author Scopus IDs: 57194603392
57209545865
55773009800
56603000600
Author Affiliations: Arora, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Singh, P., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Rani, R., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Ghosh, S., Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India
Corresponding Author: Ghosh, S.; Department of Biotechnology, Indian Institute of Technology RoorkeeIndia; email: sanjoyiitr@gmail.com
Appears in Collections:Journal Publications [BT]

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