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Please use this identifier to cite or link to this item: http://repository.iitr.ac.in/handle/123456789/13344
Title: Rice straw fermentation by Schizophyllum commune ARC-11 to produce high level of xylanase for its application in pre-bleaching
Authors: Gautam A.
Kumar A.
Bharti A.K.
Dutt D.
Published in: Journal of Genetic Engineering and Biotechnology
Abstract: Rice straw is valuable resource that has been used as substrate for cost effective production of xylanase under solid-state fermentation by a newly isolated white rot fungi, S. commune ARC-11. Out of eleven carbon sources tested, rice straw was found most effective for the induction of xylanase that produced 4288.3 IU/gds of xylanase by S. commune ARC-11. Maximum xylanase production (6721.9 IU/gds) was observed on 8th day of incubation at temperature (30 °C), initial pH (7.0) and initial moisture content (70.0%). The supplementation of ammonium sulphate (0.08% N, as available nitrogen) enhanced the xylanase production up to 8591.4 IU/gds. The xylanase production by S. commune ARC-11 was further improved by the addition of 0.10%, (w/v) of Tween-20 as surfactant. The maximum xylanase activities were found at pH 5.0 and temperature 55 °C with a longer stability (180 min) at temperature 45, 50 and 55 °C. This xylanase preparation was also evaluated for the pre-bleaching of ethanol-soda pulp from Eulaliopsis binata. An enzyme dosage of 10 IU/g of xylanase resulted maximum decrease in kappa number (14.51%) with a maximum improvement 2.9% in ISO brightness compared to control. © 2018
Citation: Journal of Genetic Engineering and Biotechnology (2018), 16(2): 693-701
URI: https://doi.org/10.1016/j.jgeb.2018.02.006
http://repository.iitr.ac.in/handle/123456789/13344
Issue Date: 2018
Publisher: Academy of Scientific Research and Technology
Keywords: Eulaliopsis binata
Pre-bleaching
Rice straw
Schizophyllum commune
Xylanase
ISSN: 1687157X
Author Scopus IDs: 57198173170
57206266862
55756871200
7005982560
Author Affiliations: Gautam, A., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247 001, India
Kumar, A., Department of Biotechnology, College of Natural and Computational Sciences, Debre Markos University, Ethiopia
Bharti, A.K., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247 001, India
Dutt, D., Department of Paper Technology, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, 247 001, India
Corresponding Author: Kumar, A.; Department of Biotechnology, College of Natural and Computational Sciences, Debre Markos UniversityEthiopia; email: amitkdpt@iitr.ac.in
Appears in Collections:Journal Publications [PT]

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